Detailed information of TA system TA087220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 39563..40122
• Replicon: plasmid unnamed1
• Accession: NZ_CP024241
• Organism: Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: COG33_RS26095
• Protein ID: WP_000421259.1
• Coordinates: 39563..39838 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: COG33_RS26100
• Protein ID: WP_001178087.1
• Coordinates: 39838..40122 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG33_RS26075	35092..35718	-	627	WP_000620425.1	ATP-binding cassette domain-containing protein	-
COG33_RS26080	35699..36484	-	786	WP_000775193.1	hypothetical protein	-
COG33_RS26085	36441..37646	-	1206	WP_000673444.1	TolC family protein	-
COG33_RS26090	37643..38779	-	1137	WP_000465104.1	hypothetical protein	-
COG33_RS26095	39563..39838	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
COG33_RS26100	39838..40122	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
COG33_RS26115	41029..42060	-	1032	WP_003964539.1	replication initiation protein	-
COG33_RS26130	43032..43295	+	264	WP_024167927.1	ash family protein	-
COG33_RS26135	43213..43500	+	288	WP_000074855.1	conjugal transfer protein TraA	-
COG33_RS26145	43942..44475	+	534	WP_001712498.1	transcription termination factor nusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltA / eltB / csvA	1..104674	104674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T87220 WP_000421259.1 NZ_CP024241:c39838-39563 [Escherichia coli O114:H49]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T87220 NZ_CP024241:c39838-39563 [Escherichia coli O114:H49]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACAAGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT87220 WP_001178087.1 NZ_CP024241:c40122-39838 [Escherichia coli O114:H49]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT87220 NZ_CP024241:c40122-39838 [Escherichia coli O114:H49]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAAATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGTCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625