Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2807610..2808241 Replicon chromosome
Accession NZ_CP024240
Organism Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag COG33_RS15030 Protein ID WP_001259385.1
Coordinates 2807966..2808241 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag COG33_RS15025 Protein ID WP_000593554.1
Coordinates 2807610..2807969 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG33_RS14995 2802722..2803195 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
COG33_RS15000 2803192..2803473 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
COG33_RS15005 2803550..2804908 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
COG33_RS15010 2804901..2806409 + 1509 WP_001298718.1 carbohydrate kinase -
COG33_RS15015 2806399..2806668 + 270 WP_000933060.1 HPr family phosphocarrier protein -
COG33_RS15020 2806700..2807560 + 861 WP_001131783.1 class II aldolase -
COG33_RS15025 2807610..2807969 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
COG33_RS15030 2807966..2808241 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
COG33_RS15035 2808314..2809438 - 1125 WP_001314210.1 ABC transporter permease -
COG33_RS15040 2809438..2812173 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
COG33_RS15045 2812170..2813237 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T87213 WP_001259385.1 NZ_CP024240:c2808241-2807966 [Escherichia coli O114:H49]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T87213 NZ_CP024240:c2808241-2807966 [Escherichia coli O114:H49]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT87213 WP_000593554.1 NZ_CP024240:c2807969-2807610 [Escherichia coli O114:H49]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT87213 NZ_CP024240:c2807969-2807610 [Escherichia coli O114:H49]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References