Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1121721..1122223 Replicon chromosome
Accession NZ_CP024240
Organism Escherichia coli O114:H49 strain 90-9280

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag COG33_RS06155 Protein ID WP_000767829.1
Coordinates 1121721..1121975 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag COG33_RS06160 Protein ID WP_001259255.1
Coordinates 1121972..1122223 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG33_RS06125 1116736..1116963 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
COG33_RS06130 1116977..1118035 - 1059 WP_000492318.1 transport protein YeeE -
COG33_RS06135 1118214..1119572 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
COG33_RS06140 1119562..1119624 - 63 WP_010723108.1 membrane protein YoeI -
COG33_RS06145 1119839..1120768 - 930 WP_000803351.1 LysR family transcriptional regulator -
COG33_RS06150 1120814..1121638 - 825 WP_000754758.1 SDR family oxidoreductase -
COG33_RS06155 1121721..1121975 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
COG33_RS06160 1121972..1122223 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
COG33_RS06165 1122506..1122556 + 51 WP_001364200.1 his operon leader peptide -
COG33_RS06175 1122702..1123601 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
COG33_RS06180 1123607..1124911 + 1305 WP_001356062.1 histidinol dehydrogenase -
COG33_RS06185 1124908..1125978 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
COG33_RS06190 1125978..1127045 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87207 WP_000767829.1 NZ_CP024240:c1121975-1121721 [Escherichia coli O114:H49]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87207 NZ_CP024240:c1121975-1121721 [Escherichia coli O114:H49]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87207 WP_001259255.1 NZ_CP024240:c1122223-1121972 [Escherichia coli O114:H49]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87207 NZ_CP024240:c1122223-1121972 [Escherichia coli O114:H49]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References