Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3028171..3028825 Replicon chromosome
Accession NZ_CP024232
Organism Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CNZ63_RS15820 Protein ID WP_000244781.1
Coordinates 3028418..3028825 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CNZ63_RS15815 Protein ID WP_000354046.1
Coordinates 3028171..3028437 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNZ63_RS15795 3024259..3025692 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
CNZ63_RS15800 3025737..3026048 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CNZ63_RS15805 3026212..3026871 + 660 WP_000250274.1 hemolysin III family protein -
CNZ63_RS15810 3026948..3027928 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
CNZ63_RS15815 3028171..3028437 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CNZ63_RS15820 3028418..3028825 + 408 WP_000244781.1 protein YgfX Toxin
CNZ63_RS15825 3028865..3029386 - 522 WP_001055874.1 flavodoxin FldB -
CNZ63_RS15830 3029498..3030394 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
CNZ63_RS15835 3030419..3031129 + 711 WP_000715224.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CNZ63_RS15840 3031135..3032868 + 1734 WP_001394741.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T87155 WP_000244781.1 NZ_CP024232:3028418-3028825 [Escherichia coli O6:H16]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T87155 NZ_CP024232:3028418-3028825 [Escherichia coli O6:H16]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT87155 WP_000354046.1 NZ_CP024232:3028171-3028437 [Escherichia coli O6:H16]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT87155 NZ_CP024232:3028171-3028437 [Escherichia coli O6:H16]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References