Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 832137..832755 Replicon chromosome
Accession NZ_CP024232
Organism Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CNZ63_RS04375 Protein ID WP_001291435.1
Coordinates 832537..832755 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID -
Locus tag CNZ63_RS04370 Protein ID WP_000344789.1
Coordinates 832137..832511 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNZ63_RS04360 827226..828419 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CNZ63_RS04365 828442..831591 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CNZ63_RS04370 832137..832511 + 375 WP_000344789.1 Hha toxicity modulator TomB Antitoxin
CNZ63_RS04375 832537..832755 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CNZ63_RS04380 832927..833514 + 588 WP_023486916.1 maltose O-acetyltransferase -
CNZ63_RS04385 833594..834064 + 471 WP_000136192.1 YlaC family protein -
CNZ63_RS04390 834228..835778 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CNZ63_RS04395 835820..836173 - 354 WP_000878143.1 DUF1428 domain-containing protein -
CNZ63_RS04405 836552..836863 + 312 WP_000409911.1 MGMT family protein -
CNZ63_RS04410 836894..837466 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87147 WP_001291435.1 NZ_CP024232:832537-832755 [Escherichia coli O6:H16]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87147 NZ_CP024232:832537-832755 [Escherichia coli O6:H16]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14569.44 Da        Isoelectric Point: 4.7395

>AT87147 WP_000344789.1 NZ_CP024232:832137-832511 [Escherichia coli O6:H16]
MDEYSPKRHDIAQLKFLCEILYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87147 NZ_CP024232:832137-832511 [Escherichia coli O6:H16]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAATCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References