Detailed information of TA system TA086950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1683678..1684383
• Replicon: chromosome
• Accession: NZ_CP024147
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A080J8D4
• Locus tag: CR539_RS08660
• Protein ID: WP_032280579.1
• Coordinates: 1683997..1684383 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CR539_RS08655
• Protein ID: WP_001280945.1
• Coordinates: 1683678..1684007 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS08640	1678835..1679746	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
CR539_RS08645	1679924..1682272	+	2349	WP_028132100.1	EAL domain-containing protein	-
CR539_RS08650	1682280..1683608	+	1329	WP_032280577.1	GGDEF domain-containing protein	-
CR539_RS08655	1683678..1684007	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CR539_RS08660	1683997..1684383	-	387	WP_032280579.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR539_RS08665	1684609..1685934	-	1326	WP_101969364.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CR539_RS08670	1686147..1686530	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CR539_RS08675	1686641..1687756	+	1116	WP_032280599.1	aldose sugar dehydrogenase YliI	-
CR539_RS08680	1687753..1688379	-	627	WP_032280600.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14350.55 Da
• Isoelectric Point: 10.0737

>T86950 WP_032280579.1 NZ_CP024147:c1684383-1683997 [Escherichia coli]
MGVYKERRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T86950 NZ_CP024147:c1684383-1683997 [Escherichia coli]
ATGGGTGTGTATAAGGAAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT86950 WP_001280945.1 NZ_CP024147:c1684007-1683678 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT86950 NZ_CP024147:c1684007-1683678 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A080J8D4