Detailed information of TA system TA086890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3171018..3171723
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CR537_RS16390
• Protein ID: WP_000539521.1
• Coordinates: 3171018..3171404 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CR537_RS16395
• Protein ID: WP_001280945.1
• Coordinates: 3171394..3171723 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS16370	3167022..3167648	+	627	WP_001295292.1	glutathione S-transferase GstB	-
CR537_RS16375	3167645..3168760	-	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
CR537_RS16380	3168871..3169254	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CR537_RS16385	3169467..3170792	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CR537_RS16390	3171018..3171404	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR537_RS16395	3171394..3171723	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CR537_RS16400	3171793..3173121	-	1329	WP_000086919.1	GGDEF domain-containing protein	-
CR537_RS16405	3173129..3175477	-	2349	Protein_3014	EAL domain-containing protein	-
CR537_RS16410	3175655..3176566	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T86890 WP_000539521.1 NZ_CP024138:3171018-3171404 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T86890 NZ_CP024138:3171018-3171404 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT86890 WP_001280945.1 NZ_CP024138:3171394-3171723 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT86890 NZ_CP024138:3171394-3171723 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6