Detailed information of TA system TA086726

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 66404..67134
• Replicon: plasmid pCh25_67
• Accession: NZ_CP024103
• Organism: Bacillus cytotoxicus strain CH_25

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: CG482_RS22900
• Protein ID: WP_094374045.1
• Coordinates: 66562..67134 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	66404..66500 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG482_RS22875	61512..61721	+	210	WP_094374055.1	DUF5511 family protein	-
CG482_RS22880	61718..63487	+	1770	WP_162533114.1	hypothetical protein	-
CG482_RS22885	63594..64574	+	981	WP_094374051.1	hypothetical protein	-
CG482_RS22890	64599..65150	+	552	WP_094374049.1	metal-dependent hydrolase	-
CG482_RS22895	65278..66300	+	1023	WP_094374047.1	DUF3991 and TOPRIM domain-containing protein	-
-	66404..66500	+	97	NuclAT_0	-	Antitoxin
-	66404..66500	+	97	NuclAT_0	-	Antitoxin
-	66404..66500	+	97	NuclAT_0	-	Antitoxin
-	66404..66500	+	97	NuclAT_0	-	Antitoxin
CG482_RS22900	66562..67134	+	573	WP_094374045.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67318	67318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22516.91 Da
• Isoelectric Point: 9.5095

>T86726 WP_094374045.1 NZ_CP024103:66562-67134 [Bacillus cytotoxicus]
MANKDNPKFYTISTKYIDYLRETDNKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDKDNPEKR
IGVLLVNNMIPVPEKECKEIDIAEKMETDPKYGNLMLKQYLFLKENMDRLSHKVEKVYKDVTVQGKPSKKQKFLKGICCD
FSTLEEKCQEYKEKNQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T86726 NZ_CP024103:66562-67134 [Bacillus cytotoxicus]
TTGGCTAATAAAGATAATCCTAAATTCTATACGATTTCAACTAAATATATAGACTATCTAAGGGAAACAGATAATAAAGT
GCCCTTTAATAAAGATGAACAGCATAGTAGACCGTATGTAGGCGTATTGGAAAAAATCAATGGTCATGATTATTTTGTTC
CTTTAACATCACGTAATGATAAAAACTTTAATAGCCAGGTCAGTGTGAAATTATTCGATAAAGATAACCCTGAAAAGCGA
ATTGGAGTTTTATTAGTCAATAACATGATTCCAGTCCCAGAAAAAGAGTGTAAAGAAATTGATATAGCAGAAAAAATGGA
GACAGATCCTAAGTATGGTAATTTGATGTTAAAACAGTATTTGTTTTTAAAAGAGAATATGGATCGACTTTCTCATAAAG
TGGAAAAAGTATATAAAGATGTAACTGTACAAGGAAAGCCATCAAAAAAACAGAAATTTCTAAAGGGAATTTGCTGTGAC
TTCTCAACATTAGAAGAAAAGTGCCAGGAATATAAAGAGAAGAATCAAGCTAGAAGAATAGCATATATGCGTCAAATGGG
GCGTGAAAGATAA

Antitoxin

Nucleotide

• Length: 97 bp

>AT86726 NZ_CP024103:66404-66500 [Bacillus cytotoxicus]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCACAAAAAAA

Structures

Antitoxin