Detailed information of TA system TA086719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1999187..2000179
• Replicon: chromosome
• Accession: NZ_CP024098
• Organism: Bacillus cytotoxicus strain CH_38

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: CG483_RS10255
• Protein ID: WP_012094283.1
• Coordinates: 1999187..1999927 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: CG483_RS10260
• Protein ID: WP_012094285.1
• Coordinates: 2000051..2000179 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CG483_RS23130	1994327..1994592	+	266	Protein_1870	hypothetical protein	-
CG483_RS10225	1994662..1995564	+	903	WP_012094278.1	ATPase	-
CG483_RS10230	1995611..1995916	-	306	Protein_1872	hypothetical protein	-
CG483_RS10235	1996111..1997415	+	1305	WP_087094924.1	DEAD/DEAH box helicase	-
CG483_RS10240	1997635..1998117	+	483	WP_012094281.1	MarR family winged helix-turn-helix transcriptional regulator	-
CG483_RS10245	1998255..1998992	+	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CG483_RS10255	1999187..1999927	+	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CG483_RS10260	2000051..2000179	+	129	WP_012094285.1	hypothetical protein	Antitoxin
CG483_RS10265	2000256..2000432	+	177	WP_087094925.1	stage III sporulation protein AA	-
CG483_RS10270	2000591..2001136	-	546	WP_087094926.1	GNAT family N-acetyltransferase	-
CG483_RS10275	2001177..2002058	-	882	WP_087094927.1	aminoglycoside 6-adenylyltransferase	-
CG483_RS10280	2002264..2003733	+	1470	WP_087094928.1	aminoacyl-histidine dipeptidase	-
CG483_RS10285	2004332..2004583	+	252	WP_012094290.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T86719 WP_012094283.1 NZ_CP024098:1999187-1999927 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T86719 NZ_CP024098:1999187-1999927 [Bacillus cytotoxicus]
TTGACTGTCACTAGCGTTCGAATTGGCTTGTTTTTTCTTGTAATCTTTTTTTTATTTCTTATTTTCTTTTATTGGAGAAA
TGAAGAACGATACGAGGAAAGAAAACAACTTATTCGAAAGACGTGGTATGGTTTATTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAACATATTTTAATGGTTGTTGCGATGGTTGTATTTGTTGATATTGCA
TTTATTTTAACGCCAAATATTTCGGAAATCTGGGGCGCAAAGTTTAGTGATATTGGTAAGACAGTTCAATCGATTAAACG
ATCGCTTATTGCTTCAAAAGCAAGAGGAGAAATATATACAACACTTATTCAAAATGTAAATCCAGCTGCATTTGGTACGA
TGGAATGGGAAACAGATGCTGAGTATGCACGCAGTTTAAATGCTTTTCTTGATACGTATGCTGAAAAAATAGGTGCGAAA
ATTGTAGTATTTGTCACAGAAGAGGAATTAAATACAAACTTCCGAGGTGTTCGATCTCAATTTAGTATTACAGTATCACC
CGAATCTATTGAACAACTGAATGAGCAAAAAGCAGTTCAAATTGAAAATGTTGGAATTATACCAGCGAAAATAATAAAAG
ACGTTTATATTATTATGGATGGACAAAAAAATAATTTGCAAGATCGTGACTTTGAAAATGTATATAATTTAACAATACAT
CATAGTTACTTTTGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT86719 WP_012094285.1 NZ_CP024098:2000051-2000179 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT86719 NZ_CP024098:2000051-2000179 [Bacillus cytotoxicus]
GTGTTAAAAGAATATAGCAATGATTTCTTTAAAGACGAAGCTGAGGATACAACAGATTTTTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTGGAAGAATTAGAAGAAGAATTGAAAAGAAGAGGATAA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7