Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5225129..5225760 Replicon chromosome
Accession NZ_CP024086
Organism Pseudomonas putida strain E46

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag CR512_RS23170 Protein ID WP_099430453.1
Coordinates 5225533..5225760 (-) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CR512_RS23165 Protein ID WP_099430452.1
Coordinates 5225129..5225536 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR512_RS23145 5221776..5223098 + 1323 WP_099430450.1 phosphatase PAP2/dual specificity phosphatase family protein -
CR512_RS23150 5223095..5223526 + 432 WP_099430451.1 hypothetical protein -
CR512_RS23155 5223537..5224160 + 624 WP_027595452.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
CR512_RS23160 5224162..5225094 + 933 WP_027595453.1 phosphatidate cytidylyltransferase -
CR512_RS23165 5225129..5225536 - 408 WP_099430452.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CR512_RS23170 5225533..5225760 - 228 WP_099430453.1 type II toxin-antitoxin system HicA family toxin Toxin
CR512_RS23175 5226036..5226242 + 207 Protein_4471 DUF861 domain-containing protein -
CR512_RS23180 5226379..5227011 - 633 WP_059183340.1 hypothetical protein -
CR512_RS23185 5227001..5230645 - 3645 WP_172960388.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8315.30 Da        Isoelectric Point: 10.5627

>T86686 WP_099430453.1 NZ_CP024086:c5225760-5225533 [Pseudomonas putida]
MRSREVIQLIEADGWYEVEVKGSHHQFRHPTKRGRVTVPHPKSDLPKGTVHSILKQAGLKQPNGSNSSSTSGDTQ

Download         Length: 228 bp

>T86686 NZ_CP024086:c5225760-5225533 [Pseudomonas putida]
ATGCGCAGTCGTGAAGTAATTCAGCTTATCGAGGCAGATGGGTGGTACGAAGTAGAGGTGAAGGGCAGCCATCACCAGTT
CAGGCACCCAACCAAAAGGGGGAGGGTGACGGTTCCCCACCCTAAGAGCGATCTGCCTAAGGGCACTGTACATAGCATCT
TGAAGCAAGCTGGGCTGAAGCAGCCTAATGGCTCCAACAGCTCAAGTACCAGTGGAGACACGCAATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14672.49 Da        Isoelectric Point: 4.7169

>AT86686 WP_099430452.1 NZ_CP024086:c5225536-5225129 [Pseudomonas putida]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGASVSQALENVQEALALHFEGLVADNEALPQAQEIDVHVANPDYQGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELAIAH

Download         Length: 408 bp

>AT86686 NZ_CP024086:c5225536-5225129 [Pseudomonas putida]
ATGAAATTTCCGGTCGTATTGCACAAAGACGCTGATTCGGACTACGGGGTGACCGTTCCTGATGTTCCTGGCTGCTTTTC
AGCAGGCGCCAGTGTCTCTCAGGCGTTGGAGAATGTTCAGGAGGCACTGGCGTTACATTTTGAAGGTCTGGTTGCCGATA
ACGAAGCGTTACCGCAGGCGCAAGAGATCGATGTTCATGTGGCTAACCCCGATTACCAAGGTGGGGTCTGGGCGGTCGTG
GACTTCGATGTGACCCCCTACCTTGGCAAGGCAGTGCGATTCAATGCGACGCTGCCGGAAAATTTGTTGCAACGGATCGA
TGAGAAGGTAAAGCGCGACCATAGGTATGCATCGCGTTCCGGATTTTTAGCATCGGCAGCGTTGCGAGAGCTGGCAATCG
CACACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References