Detailed information of TA system TA086635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 293118..293749
• Replicon: chromosome
• Accession: NZ_CP024056
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CRT55_RS02350
• Protein ID: WP_032203651.1
• Coordinates: 293118..293393 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: CRT55_RS02355
• Protein ID: WP_000593555.1
• Coordinates: 293390..293749 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS02335	288122..289189	+	1068	WP_000361467.1	HlyD family secretion protein	-
CRT55_RS02340	289186..291921	+	2736	WP_000149164.1	ribosome-associated ATPase/putative transporter RbbA	-
CRT55_RS02345	291921..293045	+	1125	WP_001216257.1	ABC transporter permease	-
CRT55_RS02350	293118..293393	+	276	WP_032203651.1	type II toxin-antitoxin system HicA family toxin	Toxin
CRT55_RS02355	293390..293749	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CRT55_RS02360	293869..294270	-	402	WP_062882695.1	nickel-responsive transcriptional regulator NikR	-
CRT55_RS02365	294276..295082	-	807	WP_062882694.1	nickel import ATP-binding protein NikE	-
CRT55_RS02370	295079..295843	-	765	WP_062882693.1	nickel import ATP-binding protein NikD	-
CRT55_RS02375	295843..296676	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
CRT55_RS02380	296673..297617	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	285360..294270	8910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10213.97 Da
• Isoelectric Point: 10.7076

>T86635 WP_032203651.1 NZ_CP024056:293118-293393 [Escherichia coli]
MRTKLQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T86635 NZ_CP024056:293118-293393 [Escherichia coli]
ATGAGAACCAAGTTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT86635 WP_000593555.1 NZ_CP024056:293390-293749 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT86635 NZ_CP024056:293390-293749 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6