Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1329742..1330361 Replicon chromosome
Accession NZ_CP023963
Organism Klebsiella aerogenes strain FDAARGOS_363

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag CRN78_RS06445 Protein ID WP_015367918.1
Coordinates 1329742..1329960 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag CRN78_RS06450 Protein ID WP_015367917.1
Coordinates 1329987..1330361 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN78_RS06415 1325815..1326075 + 261 WP_015367923.1 type B 50S ribosomal protein L31 -
CRN78_RS06420 1326078..1326218 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CRN78_RS06425 1326215..1326925 - 711 WP_015704607.1 GNAT family N-acetyltransferase -
CRN78_RS06430 1327028..1328482 + 1455 WP_015367921.1 PLP-dependent aminotransferase family protein -
CRN78_RS06435 1328457..1328921 - 465 WP_015367920.1 YlaC family protein -
CRN78_RS06440 1329047..1329610 - 564 WP_045361854.1 maltose O-acetyltransferase -
CRN78_RS06445 1329742..1329960 - 219 WP_015367918.1 hemolysin expression modulator Hha Toxin
CRN78_RS06450 1329987..1330361 - 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
CRN78_RS06455 1330834..1333980 - 3147 WP_015367916.1 multidrug efflux RND transporter permease subunit -
CRN78_RS06460 1334003..1335202 - 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1321076..1330361 9285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T86509 WP_015367918.1 NZ_CP023963:c1329960-1329742 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T86509 NZ_CP023963:c1329960-1329742 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCAGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT86509 WP_015367917.1 NZ_CP023963:c1330361-1329987 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT86509 NZ_CP023963:c1330361-1329987 [Klebsiella aerogenes]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCTCAGCTAAGATTTCTCTGCGAAACGTTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCTCAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTCCAAAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References