Detailed information of TA system TA086433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4340888..4341547
• Replicon: chromosome
• Accession: NZ_CP023956
• Organism: Serratia fonticola strain FDAARGOS_411

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CRN79_RS19485
• Protein ID: WP_098930548.1
• Coordinates: 4340888..4341241 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U2NFA4
• Locus tag: CRN79_RS19490
• Protein ID: WP_021180280.1
• Coordinates: 4341245..4341547 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN79_RS19465	4336400..4337686	+	1287	WP_098930544.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
CRN79_RS19470	4337683..4338558	+	876	WP_024528101.1	ATP-binding cassette domain-containing protein	-
CRN79_RS19475	4338555..4339271	+	717	WP_065683272.1	ABC transporter ATP-binding protein	-
CRN79_RS19480	4339443..4340630	+	1188	WP_098930546.1	sugar transporter	-
CRN79_RS19485	4340888..4341241	+	354	WP_098930548.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN79_RS19490	4341245..4341547	+	303	WP_021180280.1	XRE family transcriptional regulator	Antitoxin
CRN79_RS19495	4341691..4342551	+	861	WP_098930550.1	aldo/keto reductase family oxidoreductase	-
CRN79_RS19500	4342810..4344012	+	1203	WP_024528105.1	Bcr/CflA family multidrug efflux MFS transporter	-
CRN79_RS19505	4344085..4345029	-	945	WP_098930552.1	flavin reductase family protein	-
CRN79_RS19510	4345050..4346219	-	1170	WP_024528107.1	flavin-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13648.61 Da
• Isoelectric Point: 9.5694

>T86433 WP_098930548.1 NZ_CP023956:4340888-4341241 [Serratia fonticola]
VWEIKTTDMFDEWFSRLEDTDRASVLASLIVLREKGPQLPRPYADTVKGSRYSNMKELRVQSRGIPIRAFFAFDPRRRGI
LLCAGNKAGNEKRFYEVMIPIADREFTQFLNTSENKE

Nucleotide

• Length: 354 bp

>T86433 NZ_CP023956:4340888-4341241 [Serratia fonticola]
GTGTGGGAGATTAAAACAACGGATATGTTTGATGAGTGGTTCAGCCGGTTGGAGGACACTGACAGAGCTTCTGTGCTGGC
GTCACTCATTGTACTACGTGAAAAAGGTCCTCAGTTGCCCAGACCTTATGCCGACACGGTAAAGGGATCGCGCTACAGCA
ACATGAAGGAACTCCGCGTGCAAAGCCGAGGCATACCGATACGGGCATTCTTTGCCTTTGATCCACGTCGTCGCGGTATT
TTGCTTTGTGCTGGTAACAAGGCCGGTAATGAGAAGCGTTTTTATGAGGTGATGATCCCGATTGCCGATCGGGAGTTTAC
CCAATTTCTGAATACATCAGAGAACAAGGAGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11022.78 Da
• Isoelectric Point: 6.4749

>AT86433 WP_021180280.1 NZ_CP023956:4341245-4341547 [Serratia fonticola]
MGRTLEQILAEEKPEVVASAERIAADTLLNIHLAELRERVQKTQVEMAQALGIKQPTVAGMEKPGRDLKLSSLKRYIEAA
GGKLRLDIELPDGSHYGFVI

Nucleotide

• Length: 303 bp

>AT86433 NZ_CP023956:4341245-4341547 [Serratia fonticola]
ATGGGCAGAACACTGGAACAAATCCTGGCGGAAGAAAAACCTGAAGTAGTGGCCAGCGCTGAGCGCATTGCGGCGGATAC
GCTGCTCAATATTCACCTGGCCGAGCTTCGTGAGCGGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCGCTTGGCATCA
AACAACCCACCGTCGCGGGTATGGAAAAGCCTGGCCGCGATCTGAAGCTCTCCTCGCTCAAGCGCTATATCGAGGCTGCG
GGGGGCAAGCTGCGTCTGGATATCGAGCTGCCGGATGGTTCGCACTACGGGTTTGTTATTTAA

Structures

Antitoxin

• AlphaFold DB: U2NFA4