Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 160030..160283 Replicon plasmid unnamed2
Accession NZ_CP023942
Organism Klebsiella pneumoniae strain FDAARGOS_444

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag CRN70_RS28980 Protein ID WP_001312851.1
Coordinates 160030..160179 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 160224..160283 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN70_RS28935 155389..155804 - 416 Protein_199 IS1 family transposase -
CRN70_RS28945 156053..156454 - 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
CRN70_RS30810 156387..156644 - 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
CRN70_RS28950 156737..157390 - 654 WP_000616807.1 CPBP family intramembrane metalloprotease -
CRN70_RS28960 158329..159186 - 858 WP_032495102.1 incFII family plasmid replication initiator RepA -
CRN70_RS28965 159179..159253 - 75 WP_001375168.1 RepA leader peptide Tap -
CRN70_RS28975 159498..159746 - 249 WP_000083837.1 replication regulatory protein RepA -
CRN70_RS28980 160030..160179 - 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin Toxin
- 160224..160283 + 60 NuclAT_1 - Antitoxin
- 160224..160283 + 60 NuclAT_1 - Antitoxin
- 160224..160283 + 60 NuclAT_1 - Antitoxin
- 160224..160283 + 60 NuclAT_1 - Antitoxin
CRN70_RS28990 160484..160714 - 231 WP_001736714.1 hypothetical protein -
CRN70_RS28995 160878..161477 - 600 WP_032083981.1 hypothetical protein -
CRN70_RS29000 161863..162063 - 201 WP_015059022.1 hypothetical protein -
CRN70_RS29005 162195..162755 - 561 WP_000139328.1 fertility inhibition protein FinO -
CRN70_RS29010 162810..163556 - 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
CRN70_RS29015 163576..163737 - 162 Protein_212 DNA helicase -
CRN70_RS29020 163801..164505 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
CRN70_RS29025 164558..164623 + 66 Protein_214 helix-turn-helix domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / catA2 / blaKPC-2 / rmtB / blaTEM-1B / blaCTX-M-65 - 1..187926 187926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-45)


Sequences


Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T86401 WP_001312851.1 NZ_CP023942:c160179-160030 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T86401 NZ_CP023942:c160179-160030 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 60 bp

>AT86401 NZ_CP023942:160224-160283 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829C8K5


Antitoxin

Download structure file

References