Detailed information of TA system TA086131

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21137..21379
• Replicon: plasmid p4_4.1
• Accession: NZ_CP023827
• Organism: Escherichia coli strain 4/4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CRT43_RS25815
• Protein ID: WP_001312861.1
• Coordinates: 21137..21295 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	21339..21379 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT43_RS25770	16249..16476	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CRT43_RS25775	16564..17241	-	678	WP_001348626.1	PAS domain-containing protein	-
CRT43_RS25780	17375..17758	-	384	WP_001151566.1	relaxosome protein TraM	-
CRT43_RS25785	18089..18691	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CRT43_RS25790	18988..19809	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CRT43_RS25795	19928..20215	-	288	WP_000107535.1	hypothetical protein	-
CRT43_RS27780	20240..20446	-	207	WP_000275859.1	hypothetical protein	-
CRT43_RS27785	20359..20694	-	336	WP_013023876.1	hypothetical protein	-
CRT43_RS25815	21137..21295	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	21339..21379	-	41	NuclAT_1	-	Antitoxin
-	21339..21379	-	41	NuclAT_1	-	Antitoxin
-	21339..21379	-	41	NuclAT_1	-	Antitoxin
-	21339..21379	-	41	NuclAT_1	-	Antitoxin
-	22823..23009	-	187	NuclAT_0	-	-
-	22823..23009	-	187	NuclAT_0	-	-
-	22823..23009	-	187	NuclAT_0	-	-
-	22823..23009	-	187	NuclAT_0	-	-
CRT43_RS25825	23021..23740	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CRT43_RS25830	23737..24171	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
CRT43_RS25835	24226..26184	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27	senB	1..125877	125877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86131 WP_001312861.1 NZ_CP023827:c21295-21137 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86131 NZ_CP023827:c21295-21137 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT86131 NZ_CP023827:c21379-21339 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin