Detailed information of TA system TA085985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 134678..136218
• Replicon: plasmid pOB3b2
• Accession: NZ_CP023739
• Organism: Methylosinus trichosporium OB3b

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: CQW49_RS23095
• Protein ID: WP_099832048.1
• Coordinates: 135298..136218 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: CQW49_RS23090
• Protein ID: WP_099832047.1
• Coordinates: 134678..135301 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CQW49_RS23070	130440..131303	+	864	WP_099832043.1	patatin-like phospholipase family protein	-
CQW49_RS23075	131335..132048	-	714	WP_099832044.1	SMC-Scp complex subunit ScpB	-
CQW49_RS23080	132058..133146	-	1089	WP_099832045.1	DUF1403 family protein	-
CQW49_RS23085	133269..134348	+	1080	WP_099832046.1	tyrosine-type recombinase/integrase	-
CQW49_RS23090	134678..135301	+	624	WP_099832047.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CQW49_RS23095	135298..136218	+	921	WP_099832048.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CQW49_RS23100	136222..137136	+	915	WP_099832049.1	recombinase family protein	-
CQW49_RS23105	137177..138502	-	1326	WP_099832050.1	replication initiation protein RepC	-
CQW49_RS23110	138691..139674	-	984	WP_099832051.1	plasmid partitioning protein RepB	-
CQW49_RS23115	139671..140873	-	1203	WP_099832115.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..209102	209102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (19-61)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 33970.13 Da
• Isoelectric Point: 6.0851

>T85985 WP_099832048.1 NZ_CP023739:135298-136218 [Methylosinus trichosporium OB3b]
VTREPLKNVPASVRDRLTQRARAAGENVQLILTRYAIERLLYRLSASQHRERFVLKGAMLFSLWGPTPYRATGDLDLFGT
GNPEIEQLISVFRAIIQVEVVEDGVAFRSDTLRAEPTRPQDEYAGVRIMFDASIANARLPIQVDIGFGDAITPAPQEIEY
PSLIGMPAPKLKAYPPETVVAEKLEALVSLGVANSRMKDFYDLWAISRTFAFDGLVLTKAIRATFERRGTMLPTEIPFGL
SEAFAADPVKQTQWRAFLRRTEIAMAPEPLTQIIPSIASFLMPVLQSAADERTTPGKWPVGGPWGD

Nucleotide

• Length: 921 bp

>T85985 NZ_CP023739:135298-136218 [Methylosinus trichosporium OB3b]
GTGACACGAGAGCCGCTCAAGAATGTCCCCGCCTCCGTGCGAGACCGGCTGACCCAGCGTGCGCGGGCCGCCGGCGAGAA
TGTTCAGCTGATCCTGACCCGCTACGCGATCGAAAGGCTCCTTTACCGGCTGAGCGCTTCACAACATCGAGAACGGTTCG
TCCTGAAGGGCGCCATGCTCTTCAGCCTGTGGGGGCCGACGCCTTATCGCGCGACTGGAGACCTCGATCTTTTCGGGACA
GGCAACCCCGAGATCGAACAATTGATCTCCGTCTTTCGAGCAATCATTCAGGTCGAAGTCGTCGAAGACGGCGTCGCCTT
CCGATCCGACACTTTGCGCGCCGAGCCGACGCGACCACAAGACGAATATGCAGGCGTTCGGATCATGTTCGACGCCTCGA
TCGCCAATGCTCGGCTCCCGATCCAAGTGGACATCGGATTCGGCGACGCCATCACCCCTGCCCCGCAGGAGATCGAATAT
CCATCGCTGATCGGAATGCCGGCGCCGAAACTCAAGGCCTACCCGCCAGAGACAGTCGTCGCGGAAAAGCTCGAGGCTCT
CGTATCGCTCGGCGTGGCCAACAGCAGGATGAAGGATTTCTACGATCTCTGGGCGATCAGTCGGACCTTCGCCTTCGATG
GGCTGGTGCTGACCAAAGCGATACGGGCGACATTCGAGCGCCGCGGAACGATGCTGCCGACCGAAATTCCATTCGGACTT
TCAGAGGCGTTCGCTGCGGATCCGGTCAAGCAAACACAGTGGCGGGCCTTCCTTCGGCGCACCGAGATCGCGATGGCGCC
GGAGCCCCTGACCCAAATTATCCCGTCGATCGCCTCATTTCTGATGCCGGTCCTTCAGTCCGCCGCCGACGAACGGACCA
CTCCAGGAAAATGGCCGGTCGGCGGGCCTTGGGGAGATTGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 22820.54 Da
• Isoelectric Point: 10.5683

>AT85985 WP_099832047.1 NZ_CP023739:134678-135301 [Methylosinus trichosporium OB3b]
MQQSPTIQAKSLKELALAVARDRGIARSRDFEAAGVPRIYLQRLRDEGLLTQPGRGLYALANSQISAHHSLAEAAKSVPS
GVIGLLSALQFHELTTQLPSQVWMLLPSKAWAPRRSPVTLKILRASGEALKAGVEQQLVDRVVVPITSPAKTVADCFKYR
GKIGLDVAIEALRDCLTKKRATRDEIWRYAAIDRVQNVMRPYLEALS

Nucleotide

• Length: 624 bp

>AT85985 NZ_CP023739:134678-135301 [Methylosinus trichosporium OB3b]
ATGCAACAATCGCCCACGATCCAGGCCAAAAGCCTGAAGGAGCTCGCCCTCGCCGTCGCTCGCGACCGCGGGATAGCTCG
TTCGCGGGATTTCGAAGCGGCAGGAGTTCCGCGCATCTACCTTCAACGCCTCCGGGACGAGGGACTACTCACGCAACCCG
GAAGGGGCCTCTACGCGCTCGCCAACAGCCAAATCAGCGCGCATCACAGTCTCGCCGAGGCTGCCAAATCTGTTCCCAGC
GGGGTCATTGGACTGCTTTCGGCTCTCCAGTTCCATGAACTGACGACCCAGCTCCCGTCCCAGGTCTGGATGCTCCTGCC
TTCAAAGGCATGGGCGCCGAGGCGATCGCCCGTCACACTGAAGATTCTGCGCGCCAGTGGTGAGGCGCTGAAAGCCGGAG
TCGAGCAGCAGCTCGTCGATCGGGTGGTCGTCCCCATCACATCGCCTGCGAAAACCGTGGCCGACTGTTTCAAATATCGC
GGCAAAATCGGTCTCGACGTGGCTATCGAGGCGCTTCGGGACTGCCTGACGAAAAAGCGGGCCACGCGCGACGAAATCTG
GCGTTACGCCGCCATCGATCGCGTCCAGAATGTCATGCGGCCTTATCTGGAGGCGCTCTCGTGA