Detailed information of TA system TA085883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3784798..3785635
• Replicon: chromosome
• Accession: NZ_CP023673
• Organism: Escherichia coli strain SMN013SH2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: CP957_RS20785
• Protein ID: WP_062882216.1
• Coordinates: 3785093..3785635 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CP957_RS20780
• Protein ID: WP_001297137.1
• Coordinates: 3784798..3785109 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP957_RS20755	3779818..3780765	+	948	WP_001239454.1	cytochrome o ubiquinol oxidase subunit II	-
CP957_RS20760	3780787..3782778	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CP957_RS20765	3782768..3783382	+	615	WP_000179835.1	cytochrome o ubiquinol oxidase subunit III	-
CP957_RS20770	3783382..3783711	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CP957_RS20775	3783723..3784613	+	891	WP_000971336.1	heme o synthase	-
CP957_RS20780	3784798..3785109	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CP957_RS20785	3785093..3785635	+	543	WP_062882216.1	GNAT family N-acetyltransferase	Toxin
CP957_RS20790	3785691..3786626	-	936	WP_062882230.1	sel1 repeat family protein	-
CP957_RS20795	3787035..3788399	+	1365	WP_101974146.1	MFS transporter	-
CP957_RS20800	3788527..3789018	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CP957_RS20805	3789186..3790097	+	912	WP_032204083.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19804.08 Da
• Isoelectric Point: 8.8918

>T85883 WP_062882216.1 NZ_CP023673:3785093-3785635 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSKIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T85883 NZ_CP023673:3785093-3785635 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAAAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT85883 WP_001297137.1 NZ_CP023673:3784798-3785109 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT85883 NZ_CP023673:3784798-3785109 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCAAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3