Detailed information of TA system TA085858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3584701..3585233
• Replicon: chromosome
• Accession: NZ_CP023669
• Organism: Methylomonas koyamae strain LM6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKLM6_RS16020
• Protein ID: WP_096876301.1
• Coordinates: 3584910..3585233 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MKLM6_RS16015
• Protein ID: WP_096876775.1
• Coordinates: 3584701..3584913 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKLM6_RS16005	3580497..3583163	-	2667	WP_096876300.1	glycosyltransferase	-
MKLM6_RS16010	3583887..3584374	+	488	Protein_3169	transposase	-
MKLM6_RS16015	3584701..3584913	+	213	WP_096876775.1	antitoxin MazE family protein	Antitoxin
MKLM6_RS16020	3584910..3585233	+	324	WP_096876301.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKLM6_RS16025	3585638..3586639	+	1002	WP_197485178.1	IS110 family transposase	-
MKLM6_RS16030	3586889..3590224	-	3336	WP_064028113.1	NAD-glutamate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11454.30 Da
• Isoelectric Point: 7.2037

>T85858 WP_096876301.1 NZ_CP023669:3584910-3585233 [Methylomonas koyamae]
MRRGDLVTVTLQGDLGKPRPALLIQSDLFDAHPSVTILPVTGELRDAPLFRIPLAANEQTGLSKPSQVMVDKPQSVSRSK
IGAVIGHLDDETLLAVNRALAVFLGFA

Nucleotide

• Length: 324 bp

>T85858 NZ_CP023669:3584910-3585233 [Methylomonas koyamae]
ATGAGGCGTGGCGATCTGGTGACGGTCACGTTACAGGGCGATCTTGGCAAACCCAGGCCGGCGTTGCTGATTCAGTCCGA
CTTGTTCGACGCGCACCCGTCCGTGACGATTTTGCCGGTTACCGGCGAGTTACGCGATGCACCGTTGTTTCGAATCCCCT
TGGCGGCCAATGAACAAACCGGGCTTAGCAAGCCTTCGCAAGTGATGGTGGATAAGCCGCAATCCGTGTCGCGCAGTAAA
ATCGGCGCCGTTATCGGACATTTGGATGACGAAACCCTGTTGGCGGTCAACCGAGCATTGGCGGTATTTTTGGGGTTTGC
CTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7947.88 Da
• Isoelectric Point: 6.4446

>AT85858 WP_096876775.1 NZ_CP023669:3584701-3584913 [Methylomonas koyamae]
MSISTSARVQKHRQSLREAGLRPLQIWVPDTRRNGFADECRRQSLLIVDDAADQATCAWLEAVADHDGWQ

Nucleotide

• Length: 213 bp

>AT85858 NZ_CP023669:3584701-3584913 [Methylomonas koyamae]
ATGAGTATCAGTACTTCGGCACGTGTGCAAAAACATCGCCAGTCATTAAGGGAGGCGGGATTGCGACCCTTGCAAATTTG
GGTGCCCGATACCCGCCGTAATGGTTTTGCCGACGAATGCCGGCGCCAGTCCCTGCTGATTGTTGATGATGCTGCGGATC
AGGCGACCTGTGCGTGGTTGGAAGCGGTAGCTGACCACGACGGCTGGCAATGA