Detailed information of TA system TA083446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239380..1239948
• Replicon: chromosome
• Accession: NZ_CP023602
• Organism: Mycobacterium tuberculosis strain LE13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CP458_RS06090
• Protein ID: WP_003405865.1
• Coordinates: 1239574..1239948 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CP458_RS06085
• Protein ID: WP_003405863.1
• Coordinates: 1239380..1239577 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP458_RS06065	1235421..1236059	-	639	WP_003898730.1	lipid droplet-associated protein	-
CP458_RS06070	1236149..1237156	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CP458_RS06075	1237173..1238156	-	984	WP_003898731.1	hypothetical protein	-
CP458_RS06080	1238219..1239292	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CP458_RS06085	1239380..1239577	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP458_RS06090	1239574..1239948	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP458_RS06095	1240151..1240849	+	699	WP_003898733.1	hypothetical protein	-
CP458_RS06100	1240967..1241152	+	186	WP_003901093.1	hypothetical protein	-
CP458_RS06105	1241079..1241354	-	276	WP_003405867.1	hypothetical protein	-
CP458_RS06110	1241597..1241920	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CP458_RS06115	1241935..1242645	-	711	WP_003911399.1	hypothetical protein	-
CP458_RS21705	1242667..1242876	+	210	WP_003911400.1	hypothetical protein	-
CP458_RS06120	1242828..1243535	-	708	Protein_1161	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T83446 WP_003405865.1 NZ_CP023602:1239574-1239948 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T83446 NZ_CP023602:1239574-1239948 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT83446 WP_003405863.1 NZ_CP023602:1239380-1239577 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT83446 NZ_CP023602:1239380-1239577 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07