Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3182812..3183499 Replicon chromosome
Accession NZ_CP023588
Organism Mycobacterium tuberculosis strain TBDM425

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag CP444_RS15690 Protein ID WP_003414624.1
Coordinates 3183056..3183499 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag CP444_RS15685 Protein ID WP_003414620.1
Coordinates 3182812..3183069 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP444_RS15665 3178132..3178986 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP444_RS15670 3179042..3180205 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP444_RS15675 3180222..3181436 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP444_RS15680 3181444..3182685 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
CP444_RS15685 3182812..3183069 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
CP444_RS15690 3183056..3183499 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
CP444_RS15695 3183579..3184241 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
CP444_RS15700 3184337..3184528 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP444_RS15705 3184860..3186608 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
CP444_RS15710 3186704..3187285 + 582 WP_003414644.1 fasciclin domain-containing protein -
CP444_RS15715 3187385..3187651 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T82628 WP_003414624.1 NZ_CP023588:3183056-3183499 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T82628 NZ_CP023588:3183056-3183499 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT82628 WP_003414620.1 NZ_CP023588:3182812-3183069 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT82628 NZ_CP023588:3182812-3183069 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References