Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 696958..697604 Replicon chromosome
Accession NZ_CP023579
Organism Mycobacterium tuberculosis strain LE492

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag CP435_RS03190 Protein ID WP_003403137.1
Coordinates 696958..697371 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag CP435_RS03195 Protein ID WP_003403139.1
Coordinates 697368..697604 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP435_RS03170 693041..694591 + 1551 WP_003403119.1 MCE family protein -
CP435_RS03175 694643..695035 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
CP435_RS03180 695032..695289 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
CP435_RS03185 695472..696707 - 1236 WP_003403128.1 ATP-binding protein -
CP435_RS03190 696958..697371 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
CP435_RS03195 697368..697604 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CP435_RS03200 697708..698214 - 507 WP_003900973.1 two component system sensor kinase HK1 -
CP435_RS03205 698328..698858 - 531 WP_003917320.1 two component system sensor kinase HK2 -
CP435_RS03210 698842..699537 - 696 WP_003918099.1 two-component system response regulator TcrA -
CP435_RS03215 699660..699971 + 312 WP_003403164.1 hypothetical protein -
CP435_RS03220 700043..700993 + 951 WP_003900184.1 DUF1259 domain-containing protein -
CP435_RS03225 701234..701818 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
CP435_RS03230 701820..702530 + 711 Protein_626 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T82035 WP_003403137.1 NZ_CP023579:c697371-696958 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T82035 NZ_CP023579:c697371-696958 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT82035 WP_003403139.1 NZ_CP023579:c697604-697368 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT82035 NZ_CP023579:c697604-697368 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References