Detailed information of TA system TA081640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1108275..1108903
• Replicon: chromosome
• Accession: NZ_CP023567
• Organism: Erwinia pyrifoliae strain EpK1/15

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: CPI84_RS05350
• Protein ID: WP_004156337.1
• Coordinates: 1108275..1108493 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CPI84_RS05355
• Protein ID: WP_012668861.1
• Coordinates: 1108523..1108903 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPI84_RS05320	1104259..1104597	+	339	WP_012668866.1	P-II family nitrogen regulator	-
CPI84_RS05325	1104620..1105921	+	1302	WP_012668865.1	ammonium transporter AmtB	-
CPI84_RS05330	1105996..1106856	-	861	WP_012668864.1	acyl-CoA thioesterase II	-
CPI84_RS05335	1107079..1107636	+	558	WP_012668863.1	YbaY family lipoprotein	-
CPI84_RS05340	1107659..1107976	-	318	WP_012668862.1	MGMT family protein	-
CPI84_RS05350	1108275..1108493	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
CPI84_RS05355	1108523..1108903	-	381	WP_012668861.1	Hha toxicity modulator TomB	Antitoxin
CPI84_RS05360	1109050..1109403	-	354	WP_012668860.1	hypothetical protein	-
CPI84_RS05365	1109860..1110006	-	147	WP_012668859.1	type B 50S ribosomal protein L36	-
CPI84_RS05370	1110017..1110277	-	261	WP_012668858.1	type B 50S ribosomal protein L31	-
CPI84_RS05375	1110432..1113572	-	3141	WP_012668857.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T81640 WP_004156337.1 NZ_CP023567:c1108493-1108275 [Erwinia pyrifoliae]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T81640 NZ_CP023567:c1108493-1108275 [Erwinia pyrifoliae]
ATGACTGACAAACTTCTTACGAAAACTGACTACCTGATGCGCCTCAGACGTTGTCGTTCAATCGACACCCTGGAACGCGT
GATTGAAAAGAACAAGTATGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCCGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTTGCCGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14721.47 Da
• Isoelectric Point: 4.8834

>AT81640 WP_012668861.1 NZ_CP023567:c1108903-1108523 [Erwinia pyrifoliae]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDCDLISQIDEYL
DDTFMLFSNYGVNTHDLQRWQKSAKRLFNIFAKECLMSQVQSSHSF

Nucleotide

• Length: 381 bp

>AT81640 NZ_CP023567:c1108903-1108523 [Erwinia pyrifoliae]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCACCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTACAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACTATGAATTACAAAATCAAGCATATCGAAGACTGCGATTTGATAAGTCAGATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTATGGCGTCAATACTCATGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCCTAATGAGCCAGGTTCAATCCAGCCATTCATTTTAG

Structures

Toxin

• AlphaFold DB: A0A831EPQ0