Detailed information of TA system TA081586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1215059..1215896
• Replicon: chromosome
• Accession: NZ_CP023541
• Organism: Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CR208_RS05770
• Protein ID: WP_000227784.1
• Coordinates: 1215059..1215601 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CR208_RS05775
• Protein ID: WP_001297137.1
• Coordinates: 1215585..1215896 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR208_RS05750	1210598..1211509	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
CR208_RS05755	1211677..1212168	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
CR208_RS05760	1212296..1213660	-	1365	WP_001000978.1	MFS transporter	-
CR208_RS05765	1214068..1215003	+	936	WP_001297127.1	sel1 repeat family protein	-
CR208_RS05770	1215059..1215601	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CR208_RS05775	1215585..1215896	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CR208_RS05780	1216081..1216971	-	891	WP_000971336.1	heme o synthase	-
CR208_RS05785	1216983..1217312	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CR208_RS05790	1217312..1217926	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CR208_RS05795	1217916..1219907	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CR208_RS05800	1219929..1220876	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T81586 WP_000227784.1 NZ_CP023541:c1215601-1215059 [Escherichia coli O104:H21 str. CFSAN002236]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T81586 NZ_CP023541:c1215601-1215059 [Escherichia coli O104:H21 str. CFSAN002236]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT81586 WP_001297137.1 NZ_CP023541:c1215896-1215585 [Escherichia coli O104:H21 str. CFSAN002236]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT81586 NZ_CP023541:c1215896-1215585 [Escherichia coli O104:H21 str. CFSAN002236]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3