Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3223849..3224390 Replicon chromosome
Accession NZ_CP023531
Organism Escherichia coli strain FDAARGOS_401

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag CO703_RS17655 Protein ID WP_000615974.1
Coordinates 3224112..3224390 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CO703_RS17650 Protein ID WP_000729704.1
Coordinates 3223849..3224109 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO703_RS17625 3219632..3220417 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
CO703_RS17630 3220389..3222101 + 1713 Protein_3130 flagellar biosynthesis protein FlhA -
CO703_RS17635 3222206..3222484 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
CO703_RS17640 3222477..3222833 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CO703_RS17645 3222890..3223663 - 774 WP_000543895.1 C40 family peptidase -
CO703_RS17650 3223849..3224109 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CO703_RS17655 3224112..3224390 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CO703_RS17660 3224546..3225286 + 741 WP_001225679.1 murein L,D-transpeptidase -
CO703_RS17665 3225257..3226024 - 768 WP_000333380.1 class II glutamine amidotransferase -
CO703_RS17670 3226230..3226808 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T81536 WP_000615974.1 NZ_CP023531:3224112-3224390 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T81536 NZ_CP023531:3224112-3224390 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT81536 WP_000729704.1 NZ_CP023531:3223849-3224109 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT81536 NZ_CP023531:3223849-3224109 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References