Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 527054..527707 Replicon chromosome
Accession NZ_CP023474
Organism Brevibacillus brevis X23

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag A616_RS02890 Protein ID WP_005830205.1
Coordinates 527357..527707 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A291BDF6
Locus tag A616_RS02885 Protein ID WP_016740787.1
Coordinates 527054..527353 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A616_RS02870 523587..524993 + 1407 WP_017247294.1 hypothetical protein -
A616_RS02875 525178..525618 + 441 WP_017247295.1 hypothetical protein -
A616_RS02880 525621..526823 + 1203 WP_100169645.1 alanine racemase -
A616_RS02885 527054..527353 + 300 WP_016740787.1 CopG family ribbon-helix-helix protein Antitoxin
A616_RS02890 527357..527707 + 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A616_RS02895 527916..528344 + 429 WP_017246614.1 anti-sigma regulatory factor -
A616_RS02900 528350..529351 + 1002 WP_017246613.1 PP2C family protein-serine/threonine phosphatase -
A616_RS02905 529376..529702 + 327 WP_017246612.1 anti-sigma factor antagonist -
A616_RS02910 529702..530196 + 495 WP_017246611.1 anti-sigma B factor RsbW -
A616_RS02915 530165..530953 + 789 WP_017246610.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T81317 WP_005830205.1 NZ_CP023474:527357-527707 [Brevibacillus brevis X23]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T81317 NZ_CP023474:527357-527707 [Brevibacillus brevis X23]
GTGATTGTCAAACGTGGCGATGTATTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACAGCGCAGATTCAAAAGG
CGAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTCATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTAACTGATAAAATAACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11308.20 Da        Isoelectric Point: 10.4095

>AT81317 WP_016740787.1 NZ_CP023474:527054-527353 [Brevibacillus brevis X23]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEQEADHTLVRLVSGV

Download         Length: 300 bp

>AT81317 NZ_CP023474:527054-527353 [Brevibacillus brevis X23]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAGCGCACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAACAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A291BDF6

References