Detailed information of TA system TA081157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4577283..4578618
• Replicon: chromosome
• Accession: NZ_CP023388
• Organism: Escherichia coli strain 1105

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: CNQ47_RS23520
• Protein ID: WP_001536504.1
• Coordinates: 4577671..4578618 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: CNQ47_RS23515
• Protein ID: WP_001536505.1
• Coordinates: 4577283..4577678 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ47_RS23465	4572541..4573359	+	819	WP_096057995.1	DUF945 domain-containing protein	-
CNQ47_RS23470	4573450..4573935	+	486	WP_096057996.1	antirestriction protein	-
CNQ47_RS23480	4573950..4574426	+	477	WP_001186774.1	RadC family protein	-
CNQ47_RS23485	4574495..4574716	+	222	WP_001552746.1	DUF987 domain-containing protein	-
CNQ47_RS23490	4574790..4575167	+	378	WP_001536509.1	type IV toxin-antitoxin system YeeU family antitoxin	-
CNQ47_RS23495	4575213..4575587	+	375	WP_000854831.1	type IV toxin-antitoxin system toxin CbtA	-
CNQ47_RS23500	4575584..4576075	+	492	WP_001683023.1	hypothetical protein	-
CNQ47_RS23505	4576087..4576284	+	198	WP_021531598.1	DUF957 domain-containing protein	-
CNQ47_RS23510	4576369..4577214	+	846	Protein_4297	DUF4942 domain-containing protein	-
CNQ47_RS23515	4577283..4577678	+	396	WP_001536505.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CNQ47_RS23520	4577671..4578618	+	948	WP_001536504.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CNQ47_RS23530	4579441..4580979	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
CNQ47_RS23540	4581345..4582679	+	1335	WP_000092909.1	cadaverine/lysine antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4560636..4588792	28156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-302)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 36260.61 Da
• Isoelectric Point: 5.7165

>T81157 WP_001536504.1 NZ_CP023388:4577671-4578618 [Escherichia coli]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDRRRKIRKEIVQIITDVLHNSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
DIYSLVTELTGNGTPVTAFPCATILSTQAEKIISMMRRTAILLRNPEQQDDESLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQHPQFCNSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN

Nucleotide

• Length: 948 bp

>T81157 NZ_CP023388:4577671-4578618 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCAACGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTAATCTTTTAAAGTTACTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TGTCAAAAGCTGGTATCTCATTGAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAGGAGAAGAAAAATTCGCAAGGAAATCGTGCAAATAATCACGGATGTTCTTCATAATTCTGACATTTT
CAGCCTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAGATCCCCGTACGTTATCCCCAGACAT
TTGCCCAGGCCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAACCACTCTTCTGGAACATCCGGAAAGCAGA
GATATTTACTCACTCGTCACGGAGTTAACGGGTAATGGCACACCTGTAACAGCATTCCCCTGTGCCACCATTTTATCCAC
TCAGGCTGAAAAGATCATATCCATGATGCGAAGAACAGCCATACTTCTTCGCAATCCCGAACAACAGGATGATGAGTCCC
TGGTACGCCACATTTACGACAATTATTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCAGTACTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGACATTGAGCGCTATGGTAACCAGCACCCACAATTTTGTAACTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATGATCCTGTACACAAATTGCGTTTTCAACGATTTGTTAAACCGATGGTATTTGGTAATA
CACAGATTGACTGGGAAGAAGCCTACTCCTGTTTCAGACAAACAGCGTTGAGTGTTCTGACCAATTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14628.96 Da
• Isoelectric Point: 11.0607

>AT81157 WP_001536505.1 NZ_CP023388:4577283-4577678 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDRLNIRYSLIGATECYNKGQSTQIPVSPEFKTSPRFKRVLVVGKSKINA

Nucleotide

• Length: 396 bp

>AT81157 NZ_CP023388:4577283-4577678 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAGCGTTATGTGTTCACCCGGGATGACTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAAGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGCAACGTTATTACAGGAAAAATCATGCCTGCAGCACCTGGTGGCTCTGCCGCTGTAATCACCGAA
GCCCTGGATCGTTTGAATATCAGATACAGCTTGATCGGCGCAACAGAATGCTACAATAAAGGTCAGTCCACACAGATTCC
GGTATCTCCGGAGTTCAAAACATCTCCTCGCTTTAAAAGAGTGTTAGTTGTAGGGAAAAGTAAAATCAATGCCTGA