Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 29317..29839 Replicon plasmid p39
Accession NZ_CP023379
Organism Escherichia coli strain 127

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag CNQ50_RS26035 Protein ID WP_000220561.1
Coordinates 29317..29598 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CNQ50_RS26040 Protein ID WP_000121743.1
Coordinates 29588..29839 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ50_RS25990 25299..25844 - 546 WP_001426313.1 hypothetical protein -
CNQ50_RS28220 25839..25913 - 75 Protein_27 DNA distortion polypeptide 3 -
CNQ50_RS25995 26170..26442 - 273 WP_000167280.1 hypothetical protein -
CNQ50_RS28225 26459..26605 - 147 WP_001567307.1 hypothetical protein -
CNQ50_RS26010 26897..27226 - 330 WP_000866654.1 hypothetical protein -
CNQ50_RS26015 27320..27562 - 243 WP_000008708.1 hypothetical protein -
CNQ50_RS26020 27552..27764 - 213 WP_001446884.1 hypothetical protein -
CNQ50_RS26025 27830..28378 - 549 WP_001178641.1 hypothetical protein -
CNQ50_RS26030 28452..28967 - 516 WP_096038027.1 DnaJ domain-containing protein -
CNQ50_RS26035 29317..29598 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNQ50_RS26040 29588..29839 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CNQ50_RS26045 30902..31756 + 855 WP_096038026.1 replication initiation protein -
CNQ50_RS26050 31781..32227 + 447 WP_001074386.1 hypothetical protein -
CNQ50_RS26055 32295..32717 + 423 WP_179364589.1 DNA distortion polypeptide 3 -
CNQ50_RS26065 33255..34319 - 1065 WP_012291478.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkD / mrkC / mrkB / mrkA / mrkF 1..39598 39598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T81058 WP_000220561.1 NZ_CP023379:c29598-29317 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T81058 NZ_CP023379:c29598-29317 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT81058 WP_000121743.1 NZ_CP023379:c29839-29588 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT81058 NZ_CP023379:c29839-29588 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References