Detailed information of TA system TA081019

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56635..56889
• Replicon: plasmid p109
• Accession: NZ_CP023372
• Organism: Escherichia coli strain 1283

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CNQ51_RS24465
• Protein ID: WP_015387399.1
• Coordinates: 56683..56889 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56635..56696 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ51_RS24415	52287..52499	+	213	WP_005012601.1	hypothetical protein	-
CNQ51_RS26230	52800..52889	-	90	Protein_66	IS1 family transposase	-
CNQ51_RS24430	52944..53621	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CNQ51_RS24435	53621..53968	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CNQ51_RS24440	53988..55559	+	1572	WP_023149734.1	IS66 family transposase	-
CNQ51_RS24445	55597..56213	-	617	Protein_70	IS1 family transposase	-
CNQ51_RS24455	56314..56496	+	183	WP_000968309.1	hypothetical protein	-
-	56635..56696	-	62	NuclAT_1	-	Antitoxin
-	56635..56696	-	62	NuclAT_1	-	Antitoxin
-	56635..56696	-	62	NuclAT_1	-	Antitoxin
-	56635..56696	-	62	NuclAT_1	-	Antitoxin
CNQ51_RS24465	56683..56889	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ51_RS24470	57173..57430	+	258	WP_000083833.1	replication regulatory protein RepA	-
CNQ51_RS24480	57666..57740	+	75	WP_032336874.1	RepA leader peptide Tap	-
CNQ51_RS24485	57733..58179	+	447	Protein_75	incFII family plasmid replication initiator RepA	-
CNQ51_RS24490	58125..58607	-	483	Protein_76	VENN motif pre-toxin domain-containing protein	-
CNQ51_RS24495	59500..60720	+	1221	WP_000410951.1	arginine deiminase	-
CNQ51_RS24500	60731..61642	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	-	1..109207	109207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T81019 WP_015387399.1 NZ_CP023372:56683-56889 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T81019 NZ_CP023372:56683-56889 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT81019 NZ_CP023372:c56696-56635 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin