Detailed information of TA system TA081015

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16552..16822
• Replicon: plasmid p109
• Accession: NZ_CP023372
• Organism: Escherichia coli strain 1283

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CNQ51_RS24215
• Protein ID: WP_001312861.1
• Coordinates: 16664..16822 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	16552..16615 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ51_RS24165	12669..13234	+	566	Protein_17	class I SAM-dependent methyltransferase	-
CNQ51_RS24170	13260..13472	+	213	WP_001348622.1	hypothetical protein	-
CNQ51_RS26225	13882..14088	+	207	WP_000275856.1	hypothetical protein	-
CNQ51_RS24190	14114..14653	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
CNQ51_RS24195	14721..14954	+	234	WP_000005990.1	DUF905 family protein	-
CNQ51_RS24200	14982..15179	+	198	Protein_22	hypothetical protein	-
CNQ51_RS24205	15234..15668	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
CNQ51_RS24210	15665..16384	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CNQ51_RS26115	16396..16584	-	189	WP_001299721.1	hypothetical protein	-
-	16396..16620	+	225	NuclAT_0	-	-
-	16396..16620	+	225	NuclAT_0	-	-
-	16396..16620	+	225	NuclAT_0	-	-
-	16396..16620	+	225	NuclAT_0	-	-
-	16552..16615	-	64	-	-	Antitoxin
CNQ51_RS24215	16664..16822	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ51_RS24225	17743..18030	+	288	WP_000107535.1	hypothetical protein	-
CNQ51_RS24230	18148..18969	+	822	WP_001234426.1	DUF945 domain-containing protein	-
CNQ51_RS24235	19266..19868	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
CNQ51_RS24240	20189..20572	+	384	WP_001151524.1	relaxosome protein TraM	-
CNQ51_RS24245	20759..21448	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	-	1..109207	109207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T81015 WP_001312861.1 NZ_CP023372:16664-16822 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T81015 NZ_CP023372:16664-16822 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT81015 NZ_CP023372:c16615-16552 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin