Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4292725..4293275 Replicon chromosome
Accession NZ_CP023357
Organism Escherichia coli strain 317

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag CNQ55_RS21790 Protein ID WP_001160966.1
Coordinates 4292725..4293039 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag CNQ55_RS21795 Protein ID WP_000125568.1
Coordinates 4293042..4293275 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ55_RS21765 4288400..4289686 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CNQ55_RS21770 4289686..4290675 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CNQ55_RS21775 4290672..4291493 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CNQ55_RS21780 4291496..4291873 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CNQ55_RS21785 4291880..4292728 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CNQ55_RS21790 4292725..4293039 - 315 WP_001160966.1 CcdB family protein Toxin
CNQ55_RS21795 4293042..4293275 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CNQ55_RS21800 4293361..4293840 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CNQ55_RS21805 4294032..4295894 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
CNQ55_RS21810 4295887..4296417 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CNQ55_RS21815 4296525..4297856 - 1332 WP_001183198.1 MFS transporter -
CNQ55_RS21820 4297915..4298202 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T80887 WP_001160966.1 NZ_CP023357:c4293039-4292725 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T80887 NZ_CP023357:c4293039-4292725 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT80887 WP_000125568.1 NZ_CP023357:c4293275-4293042 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT80887 NZ_CP023357:c4293275-4293042 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References