Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1984342..1984844 Replicon chromosome
Accession NZ_CP023353
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag CNQ56_RS09920 Protein ID WP_000767826.1
Coordinates 1984590..1984844 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ56_RS09915 Protein ID WP_001259255.1
Coordinates 1984342..1984593 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS09890 1979520..1980587 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ56_RS09895 1980587..1981657 - 1071 WP_064770503.1 histidinol-phosphate transaminase -
CNQ56_RS09900 1981654..1982958 - 1305 WP_001350692.1 histidinol dehydrogenase -
CNQ56_RS09905 1982964..1983863 - 900 WP_064770504.1 ATP phosphoribosyltransferase -
CNQ56_RS09910 1984009..1984059 - 51 WP_001364200.1 his operon leader peptide -
CNQ56_RS09915 1984342..1984593 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ56_RS09920 1984590..1984844 + 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ56_RS09925 1984927..1985751 + 825 WP_000754737.1 SDR family oxidoreductase -
CNQ56_RS09930 1985797..1986726 + 930 WP_000803366.1 LysR family transcriptional regulator -
CNQ56_RS27660 1986941..1987003 + 63 WP_010723108.1 membrane protein YoeI -
CNQ56_RS09935 1986993..1988351 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CNQ56_RS09940 1988530..1989588 + 1059 WP_064770505.1 transport protein YeeE -
CNQ56_RS09945 1989602..1989829 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T80830 WP_000767826.1 NZ_CP023353:1984590-1984844 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80830 NZ_CP023353:1984590-1984844 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80830 WP_001259255.1 NZ_CP023353:1984342-1984593 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80830 NZ_CP023353:1984342-1984593 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References