Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1770447..1771085 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag CLC88_RS09405 Protein ID WP_050864084.1
Coordinates 1770447..1770623 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CLC88_RS09410 Protein ID WP_001270286.1
Coordinates 1770669..1771085 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS09385 1766066..1767280 - 1215 WP_001348055.1 BenE family transporter YdcO -
CLC88_RS09390 1767333..1767869 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CLC88_RS09395 1767942..1769903 + 1962 WP_050864083.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CLC88_RS09400 1769995..1770225 - 231 WP_000494244.1 YncJ family protein -
CLC88_RS09405 1770447..1770623 + 177 WP_050864084.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CLC88_RS09410 1770669..1771085 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CLC88_RS09415 1771164..1772570 + 1407 WP_050864085.1 PLP-dependent aminotransferase family protein -
CLC88_RS09420 1772815..1773960 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
CLC88_RS09425 1773978..1774991 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CLC88_RS09430 1774992..1775933 + 942 WP_050864086.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1764878..1766026 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6696.78 Da        Isoelectric Point: 10.6026

>T80552 WP_050864084.1 NZ_CP023165:1770447-1770623 [Escherichia coli O22:H8]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRCSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T80552 NZ_CP023165:1770447-1770623 [Escherichia coli O22:H8]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGTGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT80552 WP_001270286.1 NZ_CP023165:1770669-1771085 [Escherichia coli O22:H8]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT80552 NZ_CP023165:1770669-1771085 [Escherichia coli O22:H8]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References