Detailed information of TA system TA080396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 49688..50265
• Replicon: plasmid pCFSAN059932
• Accession: NZ_CP023051
• Organism: Listeria monocytogenes strain FDA971911-001-001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: B1S31_RS16050
• Protein ID: WP_003728465.1
• Coordinates: 49688..50029 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: B1S31_RS16055
• Protein ID: WP_003728464.1
• Coordinates: 50023..50265 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1S31_RS16035	46167..46721	-	555	WP_003728468.1	recombinase family protein	-
B1S31_RS16040	47001..47360	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
B1S31_RS16045	47360..49495	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
B1S31_RS16050	49688..50029	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B1S31_RS16055	50023..50265	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B1S31_RS16060	50326..50577	-	252	WP_003728463.1	hypothetical protein	-
B1S31_RS16065	50708..51034	+	327	WP_003728462.1	hypothetical protein	-
B1S31_RS16070	51034..51696	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
B1S31_RS16075	51715..52092	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
B1S31_RS16280	52251..52739	+	489	WP_003728520.1	hypothetical protein	-
B1S31_RS16085	52861..53109	+	249	WP_003728519.1	hypothetical protein	-
B1S31_RS16090	53110..53535	+	426	WP_003728518.1	hypothetical protein	-
B1S31_RS16095	53539..54261	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
B1S31_RS16100	54277..54993	+	717	WP_003731678.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61922	61922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T80396 WP_003728465.1 NZ_CP023051:c50029-49688 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T80396 NZ_CP023051:c50029-49688 [Listeria monocytogenes]
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TTTTTTTTTTTTTTTTTTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT80396 WP_003728464.1 NZ_CP023051:c50265-50023 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT80396 NZ_CP023051:c50265-50023 [Listeria monocytogenes]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
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TTT

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5