Detailed information of TA system TA079551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4429..4945
• Replicon: plasmid pNissle1
• Accession: NZ_CP022687
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CIW80_RS25585
• Protein ID: WP_001554709.1
• Coordinates: 4429..4713 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8H9DQ25
• Locus tag: CIW80_RS25590
• Protein ID: WP_001554710.1
• Coordinates: 4703..4945 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS25555	1..592	+	592	WP_189328174.1	replication initiation protein	-
CIW80_RS25560	582..1229	+	648	WP_024199421.1	hypothetical protein	-
CIW80_RS25565	1232..2374	+	1143	WP_001704287.1	DUF4868 domain-containing protein	-
CIW80_RS25570	2601..2927	+	327	WP_001554615.1	MobC family plasmid mobilization relaxosome protein	-
CIW80_RS25575	2917..3765	+	849	Protein_4	relaxase/mobilization nuclease domain-containing protein	-
CIW80_RS25580	4151..4384	+	234	WP_001554708.1	MbeD family mobilization/exclusion protein	-
CIW80_RS25585	4429..4713	-	285	WP_001554709.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CIW80_RS25590	4703..4945	-	243	WP_001554710.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CIW80_RS25595	5210..6106	+	897	WP_024199422.1	replication initiation protein	-
CIW80_RS25600	6096..6743	+	648	WP_024199421.1	hypothetical protein	-
CIW80_RS25605	6746..7264	+	519	Protein_10	DUF4868 domain-containing protein	-
CIW80_RS25610	7929..8357	-	429	WP_000222531.1	helix-turn-helix domain-containing protein	-
CIW80_RS26150	8515..8685	+	171	WP_001531327.1	hypothetical protein	-
CIW80_RS25615	8649..9077	+	429	WP_000534558.1	hypothetical protein	-
CIW80_RS26155	9293..9424	-	132	WP_023151671.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..11499	11499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11265.07 Da
• Isoelectric Point: 10.4971

>T79551 WP_001554709.1 NZ_CP022687:c4713-4429 [Escherichia coli Nissle 1917]
MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLRELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGK
RERSAAYDAADKRL

Nucleotide

• Length: 285 bp

>T79551 NZ_CP022687:c4713-4429 [Escherichia coli Nissle 1917]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8958.21 Da
• Isoelectric Point: 4.2821

>AT79551 WP_001554710.1 NZ_CP022687:c4945-4703 [Escherichia coli Nissle 1917]
MATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRLPFQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL

Nucleotide

• Length: 243 bp

>AT79551 NZ_CP022687:c4945-4703 [Escherichia coli Nissle 1917]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA