Detailed information of TA system TA079482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 207375..208045
• Replicon: plasmid pPR2
• Accession: NZ_CP022667
• Organism: Rhizobium leguminosarum bv. viciae strain BIHB 1217

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CHR56_RS31900
• Protein ID: WP_018246604.1
• Coordinates: 207641..208045 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A444HGU5
• Locus tag: CHR56_RS31895
• Protein ID: WP_018246605.1
• Coordinates: 207375..207644 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHR56_RS31865	202522..202962	-	441	WP_018246610.1	DMT family transporter	-
CHR56_RS31870	202959..203444	-	486	WP_026188782.1	DMT family transporter	-
CHR56_RS31875	203587..204120	+	534	WP_018246608.1	mismatch-specific DNA-glycosylase	-
CHR56_RS31880	204173..205933	+	1761	WP_018246607.1	MOSC domain-containing protein	-
CHR56_RS31885	206029..206874	+	846	WP_018517211.1	pirin family protein	-
CHR56_RS31890	206935..207153	+	219	WP_003547964.1	hypothetical protein	-
CHR56_RS31895	207375..207644	+	270	WP_018246605.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CHR56_RS31900	207641..208045	+	405	WP_018246604.1	type II toxin-antitoxin system VapC family toxin	Toxin
CHR56_RS31905	208099..208440	-	342	WP_018246603.1	hypothetical protein	-
CHR56_RS31915	209000..209560	+	561	WP_018246601.1	hypothetical protein	-
CHR56_RS40280	210378..210539	+	162	WP_018246599.1	hypothetical protein	-
CHR56_RS31920	210546..211283	-	738	WP_026188781.1	hypothetical protein	-
CHR56_RS31925	211799..212113	+	315	WP_011655201.1	helix-turn-helix domain-containing protein	-
CHR56_RS31930	212128..212481	-	354	WP_018517210.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CHR56_RS31935	212524..213045	-	522	WP_018246596.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..605219	605219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (18-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14188.56 Da
• Isoelectric Point: 5.1421

>T79482 WP_018246604.1 NZ_CP022667:207641-208045 [Rhizobium leguminosarum bv. viciae]
VSRLYMLDTNIVSELARNPMGTITRRIAEVGPDAICVSIITASELRYGCAKKGSPKLLAQIEAILGSIPVLALDLPADAE
YGGIRAELEGAGKPTGPNDLFIAAHASALGAVLVTANMGEFMRIRALQVENWLI

Nucleotide

• Length: 405 bp

>T79482 NZ_CP022667:207641-208045 [Rhizobium leguminosarum bv. viciae]
GTGAGCAGGCTTTATATGCTGGACACCAATATCGTCTCGGAGCTTGCTCGAAACCCGATGGGCACAATCACCCGACGCAT
CGCCGAGGTTGGGCCCGATGCAATTTGCGTCAGCATTATCACGGCATCGGAGTTGCGCTACGGGTGCGCCAAGAAGGGAT
CACCGAAACTTCTAGCACAGATCGAAGCCATCCTTGGCAGCATACCGGTGCTTGCGCTGGATCTGCCCGCCGATGCTGAA
TACGGTGGCATCCGGGCTGAACTGGAGGGTGCTGGCAAGCCTACCGGTCCAAATGACTTATTCATCGCTGCCCACGCCTC
TGCCCTCGGAGCAGTTCTGGTGACGGCGAACATGGGCGAGTTCATGCGTATCCGCGCTTTGCAGGTCGAAAACTGGTTGA
TTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10200.61 Da
• Isoelectric Point: 4.5610

>AT79482 WP_018246605.1 NZ_CP022667:207375-207644 [Rhizobium leguminosarum bv. viciae]
VVNSRRQEPIPQREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPVHRKNLLEVLAGLEPLGPEDQFPDVDDT
LLPVEEIDL

Nucleotide

• Length: 270 bp

>AT79482 NZ_CP022667:207375-207644 [Rhizobium leguminosarum bv. viciae]
GTGGTCAATTCCCGACGTCAAGAGCCTATTCCCCAGCGGGAGGCCAAGCTTTTCAGAAACAACAAGAGTCAGGCTGTACG
AATCCCGGCTGACTTTGAGCTACCCGGTGACAGAGTGATGATCCACCGCGACGGCGATCGGCTCATTATAGAGCCTGTGC
ACCGGAAGAACCTCCTGGAAGTTCTGGCTGGTCTGGAGCCGCTTGGTCCTGAAGATCAGTTTCCCGATGTCGACGATACC
CTGCTTCCTGTGGAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A444HGU5