Detailed information of TA system TA078657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1315964..1316621
• Replicon: chromosome
• Accession: NZ_CP022214
• Organism: Burkholderia thailandensis strain FDAARGOS_241

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0F6L6G8
• Locus tag: A8H35_RS06605
• Protein ID: WP_009910242.1
• Coordinates: 1316289..1316621 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A8H35_RS06600
• Protein ID: WP_009910243.1
• Coordinates: 1315964..1316281 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H35_RS06565	1311138..1311929	+	792	WP_009910252.1	ethanolamine ammonia-lyase subunit EutC	-
A8H35_RS06570	1311862..1312410	-	549	WP_080556799.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
A8H35_RS06575	1312376..1312936	-	561	Protein_1199	LysR family transcriptional regulator	-
A8H35_RS06580	1313016..1313981	+	966	Protein_1200	IS256 family transposase	-
A8H35_RS06585	1314095..1315123	-	1029	WP_009910245.1	site-specific integrase	-
A8H35_RS06595	1315459..1315764	-	306	WP_080556798.1	DUF3892 domain-containing protein	-
A8H35_RS06600	1315964..1316281	-	318	WP_009910243.1	helix-turn-helix domain-containing protein	Antitoxin
A8H35_RS06605	1316289..1316621	-	333	WP_009910242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8H35_RS06610	1316663..1317250	-	588	WP_038708106.1	site-specific integrase	-
A8H35_RS06615	1317598..1318374	-	777	WP_038712299.1	hypothetical protein	-
A8H35_RS06620	1318841..1320229	-	1389	WP_038712304.1	type II secretory pathway protein	-
A8H35_RS06625	1320226..1321131	-	906	WP_009910232.1	hypothetical protein	-
A8H35_RS06630	1321128..1321409	-	282	WP_025369698.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1314095..1324844	10749
	-	flank	IS/Tn	-	-	1312992..1314182	1190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-97)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12849.92 Da
• Isoelectric Point: 10.3619

>T78657 WP_009910242.1 NZ_CP022214:c1316621-1316289 [Burkholderia thailandensis]
MKFDWTVVYYNERVKRDVFAFPAGILADYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPKGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQHELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T78657 NZ_CP022214:c1316621-1316289 [Burkholderia thailandensis]
ATGAAATTCGATTGGACGGTTGTCTACTACAACGAACGGGTGAAGCGCGACGTTTTCGCGTTCCCGGCTGGGATTCTCGC
GGACTACCTCCGGCTGTTGGATCTGATGCAGGAGTTCGGGGCGGACCTGCGGATGCCGCACTCCCGAGCAATGGGTAACG
GGTTGTTTGAGTTGCGTCCCAAGGGTAGGGAAGGTATCGGGCGGGTGTTCTATTGCACGCATGTGGGGCGGCAAGTCGTT
GTGCTGCACTCCTTCGTTAAGAAGACACAGGAAACGCCGCAACATGAATTGCGGATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11264.79 Da
• Isoelectric Point: 9.9572

>AT78657 WP_009910243.1 NZ_CP022214:c1316281-1315964 [Burkholderia thailandensis]
VKRVRAEGFNPVPHTADDTARLLAGRDVKAAYDALEDEYTALRAILSARREAGLTQAQIAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKKLVISFA

Nucleotide

• Length: 318 bp

>AT78657 NZ_CP022214:c1316281-1315964 [Burkholderia thailandensis]
GTGAAGCGCGTTCGCGCTGAAGGATTCAATCCGGTTCCGCATACGGCGGACGACACGGCGCGTCTGCTTGCCGGTCGCGA
TGTCAAGGCGGCGTATGACGCTTTGGAGGATGAGTACACCGCGTTGCGCGCTATCTTGTCTGCGCGGCGCGAGGCTGGTT
TGACGCAGGCACAGATTGCGGAGCGTATGGGGACGACCGCATCGGCGGTTTCGCGGCTTGAGGCGTCTCTGTCGAGTGAA
AAGCACTCGCCGTCATTTGCGACGCTGCGGAAATATGCTGCCGCATGCGGGAAGAAGCTTGTAATTTCGTTTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6L6G8