Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 115377..115902 Replicon plasmid pM160133_p2
Accession NZ_CP022166
Organism Escherichia coli strain M160133

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag CE141_RS26895 Protein ID WP_001159868.1
Coordinates 115597..115902 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CE141_RS26890 Protein ID WP_000813634.1
Coordinates 115377..115595 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE141_RS26860 110769..111185 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
CE141_RS26865 111182..111412 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CE141_RS26870 111677..112177 + 501 WP_000528932.1 hypothetical protein -
CE141_RS26875 112190..112963 + 774 WP_000905949.1 hypothetical protein -
CE141_RS26880 113130..114263 + 1134 WP_000545984.1 DUF3800 domain-containing protein -
CE141_RS26885 114297..114809 - 513 WP_000151784.1 hypothetical protein -
CE141_RS26890 115377..115595 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CE141_RS26895 115597..115902 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
CE141_RS26900 115903..116709 + 807 WP_000016982.1 site-specific integrase -
CE141_RS26905 117461..118216 + 756 WP_000852146.1 replication initiation protein RepE -
CE141_RS26910 118804..119970 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) / sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..173624 173624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T78623 WP_001159868.1 NZ_CP022166:115597-115902 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T78623 NZ_CP022166:115597-115902 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT78623 WP_000813634.1 NZ_CP022166:115377-115595 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT78623 NZ_CP022166:115377-115595 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References