Detailed information of TA system TA078620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 43536..43912
• Replicon: plasmid pM160133_p2
• Accession: NZ_CP022166
• Organism: Escherichia coli strain M160133

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: CE141_RS26480
• Protein ID: WP_000969988.1
• Coordinates: 43730..43912 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: CE141_RS26475
• Protein ID: WP_001324224.1
• Coordinates: 43536..43733 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE141_RS28350	38796..39032	+	237	WP_001323890.1	colicin V immunity protein	-
CE141_RS26455	39010..39321	+	312	WP_001259758.1	colicin V	-
CE141_RS26460	39491..41605	-	2115	WP_113910425.1	peptidase domain-containing ABC transporter	-
CE141_RS26465	41580..42854	-	1275	WP_000489609.1	HlyD family secretion protein	-
CE141_RS26475	43536..43733	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
CE141_RS26480	43730..43912	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE141_RS26485	44011..44319	+	309	WP_000377483.1	hypothetical protein	-
CE141_RS26500	44879..45913	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) / sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..173624	173624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T78620 WP_000969988.1 NZ_CP022166:43730-43912 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T78620 NZ_CP022166:43730-43912 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT78620 WP_001324224.1 NZ_CP022166:43536-43733 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT78620 NZ_CP022166:43536-43733 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3