Detailed information of TA system TA078596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 104800..105443
• Replicon: plasmid pABWA45_1
• Accession: NZ_CP022155
• Organism: Escherichia coli strain ABWA45

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CES94_RS27535
• Protein ID: WP_088765932.1
• Coordinates: 104800..105216 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: CES94_RS27540
• Protein ID: WP_001261282.1
• Coordinates: 105213..105443 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES94_RS27500	100372..100848	+	477	WP_000043177.1	DNA starvation/stationary phase protection protein	-
CES94_RS27505	101153..101503	-	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-
CES94_RS27510	101665..103322	+	1658	Protein_134	FAD-dependent oxidoreductase	-
CES94_RS27520	103759..104463	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CES94_RS27530	104469..104702	+	234	WP_089617563.1	helix-turn-helix domain-containing protein	-
CES94_RS27535	104800..105216	-	417	WP_088765932.1	type II toxin-antitoxin system VapC family toxin	Toxin
CES94_RS27540	105213..105443	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CES94_RS29720	105400..105822	+	423	WP_177952108.1	hypothetical protein	-
CES94_RS27550	106006..106368	+	363	WP_000533745.1	hypothetical protein	-
CES94_RS27555	106386..107195	+	810	WP_000654269.1	hypothetical protein	-
CES94_RS27565	107760..109433	+	1674	WP_000026497.1	hypothetical protein	-
CES94_RS27570	109664..109930	+	267	WP_024144810.1	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..145220	145220
	-	flank	IS/Tn	-	-	103759..104463	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15164.56 Da
• Isoelectric Point: 6.4581

>T78596 WP_088765932.1 NZ_CP022155:c105216-104800 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T78596 NZ_CP022155:c105216-104800 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT78596 WP_001261282.1 NZ_CP022155:c105443-105213 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT78596 NZ_CP022155:c105443-105213 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3