Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 82938..83463 Replicon plasmid tig00000003
Accession NZ_CP021959
Organism Klebsiella pneumoniae strain AR_0139

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag AM386_RS01290 Protein ID WP_013023785.1
Coordinates 83158..83463 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag AM386_RS01285 Protein ID WP_001568025.1
Coordinates 82938..83156 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM386_RS01250 78427..78822 + 396 Protein_92 IS6-like element IS26 family transposase -
AM386_RS31030 78846..78976 - 131 Protein_93 IS5/IS1182 family transposase -
AM386_RS01255 79010..80151 - 1142 Protein_94 HlyD family secretion protein -
AM386_RS01260 80813..81691 + 879 WP_009309916.1 restriction endonuclease -
AM386_RS01265 81732..82148 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin -
AM386_RS01270 82145..82375 - 231 WP_016338373.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM386_RS01285 82938..83156 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM386_RS01290 83158..83463 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
AM386_RS01295 83632..84027 + 396 WP_017899885.1 hypothetical protein -
AM386_RS01300 84054..84368 + 315 WP_053389906.1 hypothetical protein -
AM386_RS01305 84379..85395 + 1017 WP_017899884.1 hypothetical protein -
AM386_RS01310 85593..86387 + 795 WP_004197635.1 site-specific integrase -
AM386_RS31445 86819..87121 - 303 WP_071571079.1 hypothetical protein -
AM386_RS01320 87118..87744 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 - 1..89382 89382


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T78008 WP_013023785.1 NZ_CP021959:83158-83463 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T78008 NZ_CP021959:83158-83463 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT78008 WP_001568025.1 NZ_CP021959:82938-83156 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT78008 NZ_CP021959:82938-83156 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References