Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5344769..5345394 Replicon chromosome
Accession NZ_CP021955
Organism Klebsiella pneumoniae strain AR_0107

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag AM486_RS27250 Protein ID WP_019705794.1
Coordinates 5345011..5345394 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag AM486_RS27245 Protein ID WP_004150355.1
Coordinates 5344769..5345011 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM486_RS27220 5340759..5341358 + 600 WP_004151865.1 glucose-1-phosphatase -
AM486_RS27225 5341352..5342212 + 861 WP_004146232.1 virulence factor BrkB family protein -
AM486_RS27230 5342209..5342646 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM486_RS27235 5342691..5343632 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
AM486_RS27240 5343647..5344564 - 918 WP_032430300.1 alpha/beta hydrolase -
AM486_RS27245 5344769..5345011 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
AM486_RS27250 5345011..5345394 + 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
AM486_RS27255 5345568..5346497 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
AM486_RS27260 5346494..5347129 - 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
AM486_RS27265 5347126..5348028 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T78006 WP_019705794.1 NZ_CP021955:5345011-5345394 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T78006 NZ_CP021955:5345011-5345394 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT78006 WP_004150355.1 NZ_CP021955:5344769-5345011 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT78006 NZ_CP021955:5344769-5345011 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References