Detailed information of TA system TA078004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4895525..4896165
• Replicon: chromosome
• Accession: NZ_CP021955
• Organism: Klebsiella pneumoniae strain AR_0107

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: AM486_RS25135
• Protein ID: WP_016529833.1
• Coordinates: 4895818..4896165 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM486_RS25130
• Protein ID: WP_088296393.1
• Coordinates: 4895525..4895818 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM486_RS25110	4891724..4892482	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
AM486_RS25115	4892532..4893362	-	831	WP_032428425.1	rhomboid family intramembrane serine protease GlpG	-
AM486_RS25120	4893413..4893742	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AM486_RS25125	4893947..4895455	+	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
AM486_RS25130	4895525..4895818	-	294	WP_088296393.1	XRE family transcriptional regulator	Antitoxin
AM486_RS25135	4895818..4896165	-	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM486_RS25140	4896341..4898788	-	2448	WP_002920561.1	glycogen phosphorylase	-
AM486_RS25145	4898806..4900239	-	1434	WP_088296394.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T78004 WP_016529833.1 NZ_CP021955:c4896165-4895818 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T78004 NZ_CP021955:c4896165-4895818 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10583.36 Da
• Isoelectric Point: 10.1464

>AT78004 WP_088296393.1 NZ_CP021955:c4895818-4895525 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT78004 NZ_CP021955:c4895818-4895525 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Toxin

• AlphaFold DB: W9BBY1