Detailed information of TA system TA077621

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39766..40035
• Replicon: plasmid pEC974-3
• Accession: NZ_CP021843
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CDH88_RS27780
• Protein ID: WP_001312861.1
• Coordinates: 39877..40035 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39766..39831 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS27755	35538..36119	+	582	WP_088172508.1	single-stranded DNA-binding protein	-
CDH88_RS27760	36122..36355	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CDH88_RS27765	36414..38378	+	1965	WP_079976098.1	ParB/RepB/Spo0J family partition protein	-
CDH88_RS27770	38447..38881	+	435	WP_000845894.1	conjugation system SOS inhibitor PsiB	-
CDH88_RS27775	38878..39597	+	720	WP_000116352.1	plasmid SOS inhibition protein A	-
CDH88_RS28395	39609..39797	-	189	WP_001299721.1	hypothetical protein	-
-	39609..39833	+	225	NuclAT_0	-	-
-	39609..39833	+	225	NuclAT_0	-	-
-	39609..39833	+	225	NuclAT_0	-	-
-	39609..39833	+	225	NuclAT_0	-	-
-	39766..39831	+	66	-	-	Antitoxin
CDH88_RS27780	39877..40035	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CDH88_RS28400	40330..40641	+	312	WP_198320073.1	hypothetical protein	-
CDH88_RS28595	40882..41088	+	207	WP_000547971.1	hypothetical protein	-
CDH88_RS27795	41113..41400	+	288	WP_000107535.1	hypothetical protein	-
CDH88_RS27800	41519..42340	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CDH88_RS27805	42637..43284	-	648	WP_157698281.1	transglycosylase SLT domain-containing protein	-
CDH88_RS27810	43561..43944	+	384	WP_000124979.1	relaxosome protein TraM	-
CDH88_RS27815	44135..44821	+	687	WP_000332493.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / ant(3'')-Ia / lnu(F) / sul3	-	1..78672	78672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T77621 WP_001312861.1 NZ_CP021843:39877-40035 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T77621 NZ_CP021843:39877-40035 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT77621 NZ_CP021843:39766-39831 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin