Detailed information of TA system TA077618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 50379..50980
• Replicon: plasmid pEC974-2
• Accession: NZ_CP021842
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: CDH88_RS27170
• Protein ID: WP_001216039.1
• Coordinates: 50600..50980 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: CDH88_RS27165
• Protein ID: WP_001190711.1
• Coordinates: 50379..50600 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS27115	45551..45874	+	324	WP_001175593.1	helix-turn-helix domain-containing protein	-
CDH88_RS27125	45976..46672	+	697	Protein_47	IS1 family transposase	-
CDH88_RS27145	47739..49001	+	1263	WP_069067570.1	hypothetical protein	-
CDH88_RS27150	49074..49400	+	327	Protein_49	hypothetical protein	-
CDH88_RS27155	49417..50114	-	698	Protein_50	IS1 family transposase	-
CDH88_RS27165	50379..50600	+	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CDH88_RS27170	50600..50980	+	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CDH88_RS27175	50985..51164	+	180	WP_000113018.1	hypothetical protein	-
CDH88_RS27180	51192..52235	+	1044	WP_069067567.1	DUF968 domain-containing protein	-
CDH88_RS27190	52538..52849	+	312	WP_001351993.1	phage antirepressor Ant	-
CDH88_RS27195	52882..53733	-	852	WP_000611661.1	hypothetical protein	-
CDH88_RS27200	53844..54053	-	210	WP_000874156.1	hypothetical protein	-
CDH88_RS27215	54658..54879	+	222	WP_000542338.1	hypothetical protein	-
CDH88_RS27220	54887..55918	+	1032	WP_000067537.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / qacE / aadA2 / dfrA12	-	1..92385	92385

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T77618 WP_001216039.1 NZ_CP021842:50600-50980 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T77618 NZ_CP021842:50600-50980 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT77618 WP_001190711.1 NZ_CP021842:50379-50600 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT77618 NZ_CP021842:50379-50600 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA