Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2546798..2547339 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name yafQ Uniprot ID A0A246NT41
Locus tag CDH88_RS12635 Protein ID WP_000615977.1
Coordinates 2546798..2547076 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A7B3MMA0
Locus tag CDH88_RS12640 Protein ID WP_021576987.1
Coordinates 2547079..2547339 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS12620 2544380..2544958 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CDH88_RS12625 2545164..2545931 + 768 WP_000333380.1 class II glutamine amidotransferase -
CDH88_RS12630 2545902..2546642 - 741 WP_001225679.1 murein L,D-transpeptidase -
CDH88_RS12635 2546798..2547076 - 279 WP_000615977.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CDH88_RS12640 2547079..2547339 - 261 WP_021576987.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CDH88_RS12645 2547549..2548298 + 750 WP_000056850.1 C40 family peptidase -
CDH88_RS12650 2548474..2548971 + 498 WP_000006266.1 REP-associated tyrosine transposase RayT -
CDH88_RS12655 2549180..2551273 - 2094 WP_033815253.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10842.67 Da        Isoelectric Point: 10.0702

>T77599 WP_000615977.1 NZ_CP021840:c2547076-2546798 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNILPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T77599 NZ_CP021840:c2547076-2546798 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.78 Da        Isoelectric Point: 7.0129

>AT77599 WP_021576987.1 NZ_CP021840:c2547339-2547079 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNKLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT77599 NZ_CP021840:c2547339-2547079 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATAAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NT41

Antitoxin

Source ID Structure
AlphaFold DB A0A7B3MMA0

References