Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2319248..2319798 Replicon chromosome
Accession NZ_CP021840
Organism Escherichia coli strain EC974

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag CDH88_RS11585 Protein ID WP_001160974.1
Coordinates 2319484..2319798 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CDH88_RS11580 Protein ID WP_000125566.1
Coordinates 2319248..2319481 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDH88_RS11555 2314319..2314606 + 288 WP_000203741.1 ferredoxin-like protein FixX -
CDH88_RS11560 2314664..2315995 + 1332 WP_001183198.1 MFS transporter -
CDH88_RS11565 2316106..2316636 + 531 WP_000600729.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CDH88_RS11570 2316629..2318491 + 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
CDH88_RS11575 2318683..2319162 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
CDH88_RS11580 2319248..2319481 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CDH88_RS11585 2319484..2319798 + 315 WP_001160974.1 CcdB family protein Toxin
CDH88_RS11590 2319795..2320643 - 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CDH88_RS11595 2320650..2321027 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CDH88_RS11600 2321030..2321851 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CDH88_RS11605 2321848..2322837 - 990 WP_000241272.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CDH88_RS11610 2322837..2324123 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T77598 WP_001160974.1 NZ_CP021840:2319484-2319798 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T77598 NZ_CP021840:2319484-2319798 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT77598 WP_000125566.1 NZ_CP021840:2319248-2319481 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT77598 NZ_CP021840:2319248-2319481 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References