Detailed information of TA system TA077593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1094761..1095561
• Replicon: chromosome
• Accession: NZ_CP021840
• Organism: Escherichia coli strain EC974

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: CDH88_RS05690
• Protein ID: WP_000342448.1
• Coordinates: 1094761..1095288 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: CDH88_RS05695
• Protein ID: WP_001277109.1
• Coordinates: 1095295..1095561 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDH88_RS05660	1089836..1090603	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
CDH88_RS05665	1090600..1091877	-	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
CDH88_RS05670	1091874..1092800	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
CDH88_RS05675	1092848..1093957	-	1110	WP_088172235.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
CDH88_RS05685	1094381..1094764	+	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
CDH88_RS05690	1094761..1095288	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
CDH88_RS05695	1095295..1095561	-	267	WP_001277109.1	DUF1778 domain-containing protein	Antitoxin
CDH88_RS05700	1095711..1096814	-	1104	WP_001022009.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
CDH88_RS05705	1097085..1097939	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
CDH88_RS05710	1098184..1099242	-	1059	WP_001042006.1	permease-like cell division protein FtsX	-
CDH88_RS05715	1099235..1099903	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T77593 WP_000342448.1 NZ_CP021840:c1095288-1094761 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T77593 NZ_CP021840:c1095288-1094761 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT77593 WP_001277109.1 NZ_CP021840:c1095561-1095295 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT77593 NZ_CP021840:c1095561-1095295 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A7U9LUN0