Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 350242..350822 Replicon plasmid psymA
Accession NZ_CP021813
Organism Sinorhizobium meliloti strain M270

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag CDO26_RS19970 Protein ID WP_010968151.1
Coordinates 350439..350822 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag CDO26_RS19965 Protein ID WP_013851145.1
Coordinates 350242..350442 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO26_RS19930 (CDO26_19935) 345546..346658 + 1113 WP_088197699.1 histidinol-phosphate transaminase -
CDO26_RS19940 (CDO26_19945) 347225..347389 - 165 WP_014531164.1 hypothetical protein -
CDO26_RS19945 (CDO26_19950) 347465..348193 - 729 WP_088197701.1 class I SAM-dependent methyltransferase -
CDO26_RS39930 348232..348756 - 525 WP_272951510.1 DUF1127 domain-containing protein -
CDO26_RS19955 (CDO26_19960) 348637..349473 + 837 WP_013851144.1 helix-turn-helix transcriptional regulator -
CDO26_RS19960 (CDO26_19965) 349550..349951 - 402 WP_014531161.1 GFA family protein -
CDO26_RS19965 (CDO26_19970) 350242..350442 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CDO26_RS19970 (CDO26_19975) 350439..350822 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO26_RS19980 (CDO26_19980) 351184..353064 - 1881 WP_088197703.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
CDO26_RS19985 (CDO26_19985) 353299..355443 + 2145 WP_167693516.1 caspase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1454983 1454983


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T77499 WP_010968151.1 NZ_CP021813:350439-350822 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T77499 NZ_CP021813:350439-350822 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT77499 WP_013851145.1 NZ_CP021813:350242-350442 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT77499 NZ_CP021813:350242-350442 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References