Detailed information of TA system TA077493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 683774..684428
• Replicon: chromosome
• Accession: NZ_CP021812
• Organism: Sinorhizobium meliloti strain M270

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDO26_RS03495
• Protein ID: WP_088196891.1
• Coordinates: 684240..684428 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: CDO26_RS03490
• Protein ID: WP_010969659.1
• Coordinates: 683774..684235 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO26_RS03465 (CDO26_03460)	679909..680352	+	444	WP_003529999.1	TIGR02594 family protein	-
CDO26_RS03470 (CDO26_03465)	680503..681609	+	1107	WP_088196890.1	endonuclease	-
CDO26_RS03475 (CDO26_03470)	681612..682361	+	750	WP_010969660.1	NUDIX hydrolase	-
CDO26_RS03480 (CDO26_03475)	682538..683455	+	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
CDO26_RS03490 (CDO26_03485)	683774..684235	-	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO26_RS03495 (CDO26_03490)	684240..684428	-	189	WP_088196891.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO26_RS03500 (CDO26_03495)	684605..685375	+	771	WP_010969658.1	Stf0 family sulphotransferase	-
CDO26_RS03505 (CDO26_03500)	685556..686527	+	972	WP_017265202.1	glycosyltransferase family 2 protein	-
CDO26_RS03510 (CDO26_03505)	686551..687861	-	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6962.14 Da
• Isoelectric Point: 11.0889

>T77493 WP_088196891.1 NZ_CP021812:c684428-684240 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKQPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T77493 NZ_CP021812:c684428-684240 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACAACCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT77493 WP_010969659.1 NZ_CP021812:c684235-683774 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT77493 NZ_CP021812:c684235-683774 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Antitoxin

• AlphaFold DB: Q92NX0