Detailed information of TA system TA077483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3612680..3613298
• Replicon: chromosome
• Accession: NZ_CP021808
• Organism: Sinorhizobium meliloti strain Rm41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: CDO27_RS18015
• Protein ID: WP_010969011.1
• Coordinates: 3613002..3613298 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDO27_RS18010
• Protein ID: WP_010969010.1
• Coordinates: 3612680..3612988 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO27_RS17970	3608495..3608731	+	237	WP_153444851.1	hypothetical protein	-
CDO27_RS17980	3608913..3609278	+	366	WP_015007420.1	hypothetical protein	-
CDO27_RS36690	3610863..3611033	-	171	WP_080599795.1	hypothetical protein	-
CDO27_RS17995	3611126..3612100	+	975	WP_051032088.1	non-homologous end-joining DNA ligase	-
CDO27_RS18005	3612439..3612690	-	252	WP_042708639.1	hypothetical protein	-
CDO27_RS18010	3612680..3612988	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
CDO27_RS18015	3613002..3613298	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDO27_RS18020	3613346..3613651	-	306	WP_003527305.1	hypothetical protein	-
CDO27_RS18025	3613979..3615016	+	1038	WP_003527304.1	porin	-
CDO27_RS18030	3615126..3616193	-	1068	WP_015007424.1	site-specific integrase	-
CDO27_RS18035	3616234..3617283	-	1050	WP_013844229.1	porin	-
CDO27_RS18040	3617497..3617985	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3552156..3620195	68039

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T77483 WP_010969011.1 NZ_CP021808:c3613298-3613002 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T77483 NZ_CP021808:c3613298-3613002 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT77483 WP_010969010.1 NZ_CP021808:c3612988-3612680 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT77483 NZ_CP021808:c3612988-3612680 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61